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Free access | 10.1172/JCI109943

Transferrin Binding to Peripheral Blood Lymphocytes Activated by Phytohemagglutinin Involves a Specific Receptor: LIGAND INTERACTION

Robert M. Galbraith, Phillip Werner, Philippe Arnaud, and Gillian M. P. Galbraith

Department of Basic, Medical University of South Carolina, Charleston, South Carolina 29403

Department of Clinical Immunology and Microbiology, Medical University of South Carolina, Charleston, South Carolina 29403

Department of Pathology, Medical University of South Carolina, Charleston, South Carolina 29403

Department of Obstetrics and Gynecology, Medical University of South Carolina, Charleston, South Carolina 29403

Find articles by Galbraith, R. in: PubMed | Google Scholar

Department of Basic, Medical University of South Carolina, Charleston, South Carolina 29403

Department of Clinical Immunology and Microbiology, Medical University of South Carolina, Charleston, South Carolina 29403

Department of Pathology, Medical University of South Carolina, Charleston, South Carolina 29403

Department of Obstetrics and Gynecology, Medical University of South Carolina, Charleston, South Carolina 29403

Find articles by Werner, P. in: PubMed | Google Scholar

Department of Basic, Medical University of South Carolina, Charleston, South Carolina 29403

Department of Clinical Immunology and Microbiology, Medical University of South Carolina, Charleston, South Carolina 29403

Department of Pathology, Medical University of South Carolina, Charleston, South Carolina 29403

Department of Obstetrics and Gynecology, Medical University of South Carolina, Charleston, South Carolina 29403

Find articles by Arnaud, P. in: PubMed | Google Scholar

Department of Basic, Medical University of South Carolina, Charleston, South Carolina 29403

Department of Clinical Immunology and Microbiology, Medical University of South Carolina, Charleston, South Carolina 29403

Department of Pathology, Medical University of South Carolina, Charleston, South Carolina 29403

Department of Obstetrics and Gynecology, Medical University of South Carolina, Charleston, South Carolina 29403

Find articles by Galbraith, G. in: PubMed | Google Scholar

Published November 1, 1980 - More info

Published in Volume 66, Issue 5 on November 1, 1980
J Clin Invest. 1980;66(5):1135–1143. https://doi.org/10.1172/JCI109943.
© 1980 The American Society for Clinical Investigation
Published November 1, 1980 - Version history
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Abstract

Immunohistological studies have indicated that membrane sites binding transferrin are present upon activated human peripheral blood lymphocytes. In this study, we have investigated transferrin uptake in human lymphocytes exposed to phytohemagglutinin (PHA), by quantitative radiobinding and immunofluorescence in parallel. In stimulated lymphocytes, binding was maximal after a 30-min incubation, being greatest at 37°C, and greater at 22°C than at 4°C. Although some shedding and endocytosis of transferrin occurred at 22° and 37°C, these factors, and resulting synthesis of new sites, did not affect measurement of binding which was found to be saturable, reversible, and specific for transferrin (Ka 0.5-2.5 × 108 M−1). Binding was greater after a 48-h exposure to PHA than after 24 h, and was maximal at 66 h. Sequential Scatchard analysis revealed no significant elevation in affinity of interaction. However, although the total number of receptors increased, the proportion of cells in which binding of ligand was detected immunohistologically increased in parallel, and after appropriate correction, the cellular density of receptors remained relatively constant throughout (60,000-80,000 sites/cell). Increments in binding during the culture period were thus due predominantly to expansion of a population of cells bearing receptors. Similar differences in binding were apparent upon comparison of cells cultured in different doses of PHA, and in unstimulated cells binding was negligible. Transferrin receptors appear, therefore, to be readily detectable only upon lymphocytes that have been activated.

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