Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
Top
  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal
  • Top
  • Abstract
  • Version history
  • Article usage
  • Citations to this article

Advertisement

Free access | 10.1172/JCI109817

Protection of Human Neutrophils by Endogenous Catalase: STUDIES WITH CELLS FROM CATALASE-DEFICIENT INDIVIDUALS

Dirk Roos, Ron S. Weening, Sonja R. Wyss, and Hugo E. Aebi

Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Binnengasthuis, University of Amsterdam, Amsterdam, Netherlands

Laboratory for Experimental and Clinical Immunology, and Pediatric Clinic, Binnengasthuis, University of Amsterdam, Amsterdam, Netherlands

Medizinisch-chemisches Institut der Universität Bern, Bern, Switzerland

Find articles by Roos, D. in: PubMed | Google Scholar

Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Binnengasthuis, University of Amsterdam, Amsterdam, Netherlands

Laboratory for Experimental and Clinical Immunology, and Pediatric Clinic, Binnengasthuis, University of Amsterdam, Amsterdam, Netherlands

Medizinisch-chemisches Institut der Universität Bern, Bern, Switzerland

Find articles by Weening, R. in: PubMed | Google Scholar

Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Binnengasthuis, University of Amsterdam, Amsterdam, Netherlands

Laboratory for Experimental and Clinical Immunology, and Pediatric Clinic, Binnengasthuis, University of Amsterdam, Amsterdam, Netherlands

Medizinisch-chemisches Institut der Universität Bern, Bern, Switzerland

Find articles by Wyss, S. in: PubMed | Google Scholar

Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Binnengasthuis, University of Amsterdam, Amsterdam, Netherlands

Laboratory for Experimental and Clinical Immunology, and Pediatric Clinic, Binnengasthuis, University of Amsterdam, Amsterdam, Netherlands

Medizinisch-chemisches Institut der Universität Bern, Bern, Switzerland

Find articles by Aebi, H. in: PubMed | Google Scholar

Published June 1, 1980 - More info

Published in Volume 65, Issue 6 on June 1, 1980
J Clin Invest. 1980;65(6):1515–1522. https://doi.org/10.1172/JCI109817.
© 1980 The American Society for Clinical Investigation
Published June 1, 1980 - Version history
View PDF
Abstract

To investigate the importance of catalase as a protecting enzyme against oxidative damage in phagocytic leukocytes, we have tested the functional capacity of neutrophils from two individuals homozygous for Swiss-type acatalasemia and from two individuals heterozygous for this deficiency. In the former cells, 25-30% of residual activity of catalase was present. In the latter cells, the values were close to normal.

Chemotaxis towards casein, release of lysosomal enzymes and hydrogen peroxide during phagocytosis of zymosan, and intracellular killing of Staphylococcus aureus were normal in all cells tested. Inhibition of heme enzymes with azide (2 mM) enhanced the respiration and hexose monophosphate shunt activity of normal, but not of homozygous acatalasemic, neutrophils. This indicates that the enhancement in normal cells is, at least in part, due to catalase inhibition.

After 15 min preincubation with an H2O2-generating system (glucose plus glucose oxidase), the respiratory response to zymosan phagocytosis was strongly depressed in the homozygous acatalasemic and in normal, azide-treated neutrophils, but not in normal, untreated cells. Under these conditions, the release of lysosomal enzymes was depressed and that of lactate dehydrogenase enhanced, in catalase-deficient and in catalase-inhibited, but not in normal, neutrophils. During prolonged incubation with the H2O2-generating system (30-60 min), the reduction level of intracellular glutathione remained high and the hexose monophosphate shunt continued to operate normally in all cells tested. Thus, although the function of neutrophils without catalase activity was depressed by extracellular hydrogen peroxide, the H2O2 degradation via the glutathione redox system remained operative.

The results indicate that the glutathione redox system by itself efficiently protects phagocytosing neutrophils against their own oxidative products. During heavy external oxidative stress, however, both catalase and the glutathione redox system are needed for adequate protection.

Browse pages

Click on an image below to see the page. View PDF of the complete article

icon of scanned page 1515
page 1515
icon of scanned page 1516
page 1516
icon of scanned page 1517
page 1517
icon of scanned page 1518
page 1518
icon of scanned page 1519
page 1519
icon of scanned page 1520
page 1520
icon of scanned page 1521
page 1521
icon of scanned page 1522
page 1522
Version history
  • Version 1 (June 1, 1980): No description

Article tools

  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal

Metrics

  • Article usage
  • Citations to this article

Go to

  • Top
  • Abstract
  • Version history
Advertisement
Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts