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Free access | 10.1172/JCI109626

Generation of Hydroxyl Radical by Enzymes, Chemicals, and Human Phagocytes In Vitro: DETECTION WITH THE ANTI-INFLAMMATORY AGENT, DIMETHYL SULFOXIDE

John E. Repine, John W. Eaton, M. W. Anders, John R. Hoidal, and Richard B. Fox

Department of Medicine, Webb-Waring Lung Institute, University of Colorado Health Sciences Center, Denver, Colorado 80262

Department of Pediatrics, Webb-Waring Lung Institute, University of Colorado Health Sciences Center, Denver, Colorado 80262

Department of Laboratory Medicine

Department of Pathology and Pharmacology, University of Minnesota Health Sciences Center, Minneapolis, Minnesota 55455

Find articles by Repine, J. in: PubMed | Google Scholar

Department of Medicine, Webb-Waring Lung Institute, University of Colorado Health Sciences Center, Denver, Colorado 80262

Department of Pediatrics, Webb-Waring Lung Institute, University of Colorado Health Sciences Center, Denver, Colorado 80262

Department of Laboratory Medicine

Department of Pathology and Pharmacology, University of Minnesota Health Sciences Center, Minneapolis, Minnesota 55455

Find articles by Eaton, J. in: PubMed | Google Scholar

Department of Medicine, Webb-Waring Lung Institute, University of Colorado Health Sciences Center, Denver, Colorado 80262

Department of Pediatrics, Webb-Waring Lung Institute, University of Colorado Health Sciences Center, Denver, Colorado 80262

Department of Laboratory Medicine

Department of Pathology and Pharmacology, University of Minnesota Health Sciences Center, Minneapolis, Minnesota 55455

Find articles by Anders, M. in: PubMed | Google Scholar

Department of Medicine, Webb-Waring Lung Institute, University of Colorado Health Sciences Center, Denver, Colorado 80262

Department of Pediatrics, Webb-Waring Lung Institute, University of Colorado Health Sciences Center, Denver, Colorado 80262

Department of Laboratory Medicine

Department of Pathology and Pharmacology, University of Minnesota Health Sciences Center, Minneapolis, Minnesota 55455

Find articles by Hoidal, J. in: PubMed | Google Scholar

Department of Medicine, Webb-Waring Lung Institute, University of Colorado Health Sciences Center, Denver, Colorado 80262

Department of Pediatrics, Webb-Waring Lung Institute, University of Colorado Health Sciences Center, Denver, Colorado 80262

Department of Laboratory Medicine

Department of Pathology and Pharmacology, University of Minnesota Health Sciences Center, Minneapolis, Minnesota 55455

Find articles by Fox, R. in: PubMed | Google Scholar

Published December 1, 1979 - More info

Published in Volume 64, Issue 6 on December 1, 1979
J Clin Invest. 1979;64(6):1642–1651. https://doi.org/10.1172/JCI109626.
© 1979 The American Society for Clinical Investigation
Published December 1, 1979 - Version history
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Abstract

Methane (CH4) production from the anti-inflammatory agent, dimethyl sulfoxide (DMSO), was used to measure ·OH from chemical reactions or human phagocytes. Reactions producing ·OH (xanthine/xanthine oxidase or Fe++/EDTA/H2O2) generated CH4 from DMSO, whereas reactions yielding primarily O-2̇ or H2O2 failed to produce CH4. Neutrophils (PMN), monocytes, and alveolar macrophages also produced CH4 from DMSO. Mass spectroscopy using d6-DMSO showed formation of d3-CH4 indicating that CH4 was derived from DMSO. Methane generation by normal but not chronic granulomatous disease or heat-killed phagocytes increased after stimulation with opsonized zymosan particles or the chemical, phorbol myristate acetate. Methane production from DMSO increased as the number of stimulated PMN was increased and the kinetics of CH4 production approximated other metabolic activities of stimulated PMN. Methane production from stimulated phagocytes and DMSO was markedly decreased by purportedly potent ·OH scavengers (thiourea or tryptophane) and diminished to lesser degrees by weaker ·OH scavengers (mannitol, ethanol, or sodium benzoate). Superoxide dismutase or catalase also decreased CH4 production but urea, albumin, inactivated superoxide dismutase, or boiled catalase had no appreciable effect. The results suggest that the production of CH4 from DMSO may reflect release of ·OH from both chemical systems and phagocytic cells. Interaction of the nontoxic, highly permeable DMSO with ·OH may explain the anti-inflammatory actions of DMSO and provide a useful measurement of ·OH in vitro and in vivo.

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