Zymographic and reverse zymographic analysis of BAL fluid. (a)
Zymographic analysis of wild-type and null BAL at 2 and 5 months of age. No
differences in MMP-9 (105 kDa) or MMP-2 (72 kDa) abundance or activation
(asterisks) were noted. Incubation of a sample lane with a synthetic MMP
inhibitor, GM6001, identified which bands represented MMP gelatinolytic
activities. (b) Reverse zymographic analysis demonstrated there was
no compensation in null BAL by upregulation of either TIMP-1 or TIMP-2.
(c) Parallel Coomassie-stained SDS-PAGE gel revealed the bands in
b were gelatin-protecting bands and not Coomassie-staining
bands in the BAL. Panel c also shows approximately equal loading
between samples.