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Free access | 10.1172/JCI108842

Clinical Relevance of Circulating Immune Complexes in Human Leukemia: ASSOCIATION IN ACUTE LEUKEMIA OF THE PRESENCE OF IMMUNE COMPLEXES WITH UNFAVORABLE PROGNOSIS

Nicole A. Carpentier, Ghislaine T. Lange, Denis M. Fiere, Gilbert J. Fournie, Paul-Henri Lambert, and Peter A. Miescher

World Health Organization Immunology Research and Training Center, University of Geneva, 1211 Geneva 4, Switzerland

Blood Transfusion Center and Department of Medicine, University of Geneva, 1211 Geneva 4, Switzerland

Find articles by Carpentier, N. in: PubMed | Google Scholar

World Health Organization Immunology Research and Training Center, University of Geneva, 1211 Geneva 4, Switzerland

Blood Transfusion Center and Department of Medicine, University of Geneva, 1211 Geneva 4, Switzerland

Find articles by Lange, G. in: PubMed | Google Scholar

World Health Organization Immunology Research and Training Center, University of Geneva, 1211 Geneva 4, Switzerland

Blood Transfusion Center and Department of Medicine, University of Geneva, 1211 Geneva 4, Switzerland

Find articles by Fiere, D. in: PubMed | Google Scholar

World Health Organization Immunology Research and Training Center, University of Geneva, 1211 Geneva 4, Switzerland

Blood Transfusion Center and Department of Medicine, University of Geneva, 1211 Geneva 4, Switzerland

Find articles by Fournie, G. in: PubMed | Google Scholar

World Health Organization Immunology Research and Training Center, University of Geneva, 1211 Geneva 4, Switzerland

Blood Transfusion Center and Department of Medicine, University of Geneva, 1211 Geneva 4, Switzerland

Find articles by Lambert, P. in: PubMed | Google Scholar

World Health Organization Immunology Research and Training Center, University of Geneva, 1211 Geneva 4, Switzerland

Blood Transfusion Center and Department of Medicine, University of Geneva, 1211 Geneva 4, Switzerland

Find articles by Miescher, P. in: PubMed | Google Scholar

Published October 1, 1977 - More info

Published in Volume 60, Issue 4 on October 1, 1977
J Clin Invest. 1977;60(4):874–884. https://doi.org/10.1172/JCI108842.
© 1977 The American Society for Clinical Investigation
Published October 1, 1977 - Version history
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Abstract

The occurrence of circulating immune complexes was investigated in 467 serum samples from 230 leukemia patients using the [125I]Clq-binding test. There was an increased serum [125I]Clq-binding activity in 40% of patients with acute myeloid leukemia, 23% with acute lymphatic leukemia, 46% in blastic crisis of chronic myeloid leukemia, 12% with chronic lymphatic leukemia, and 13% with chronic myeloid leukemia. In 48 patients, serum was also tested for soluble immune complexes by the Raji cell radioassay; the correlation between results of the two tests was significant. The Clq-binding material had properties identical with those of immune complexes. It sedimented as 14-28s material on sucrose density gradient. It contained IgG which could be dissociated at acid pH. Its Clq-binding properties could be removed after passage through anti-IgG immuno-absorbant or after a mild reduction-alkylation treatment, but were not sensitive to deoxyribonuclease treatment. Circulating immune complexes were found most commonly during the blastic stage of leukemia.

Remission took place in 75.4% of patients with no detectable circulating immune complexes at the onset of acute leukemia, but in only 32.7% of those with detected complexes during this period. Median survival times of the former group of patients were more than 18 mo in acute myeloid leukemia and acute lymphatic leukemia and more than 8½ mo in blastic crisis of chronic myeloid leukemia. The corresponding median survival times in the latter patient group were 64, 135, and 90 days. These findings were unrelated to prognostic features already known.

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