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Free access | 10.1172/JCI106022

Permeability-increasing activity in hereditary angioneurotic edema plasma: II. Mechanism of formation and partial characterization

Virginia H. Donaldson, Oscar D. Ratnoff, Wilmar Dias Da Silva, and Fred S. Rosen

Shriners Burns Institute and Department of Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio 45219

laboratories of the Research Division of St. Vincent Charity Hospital, Cleveland, Ohio

Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106

Department of Pediatrics, Harvard Medical School and Children's Hospital, Boston, Massachusetts 02115

Find articles by Donaldson, V. in: PubMed | Google Scholar

Shriners Burns Institute and Department of Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio 45219

laboratories of the Research Division of St. Vincent Charity Hospital, Cleveland, Ohio

Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106

Department of Pediatrics, Harvard Medical School and Children's Hospital, Boston, Massachusetts 02115

Find articles by Ratnoff, O. in: PubMed | Google Scholar

Shriners Burns Institute and Department of Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio 45219

laboratories of the Research Division of St. Vincent Charity Hospital, Cleveland, Ohio

Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106

Department of Pediatrics, Harvard Medical School and Children's Hospital, Boston, Massachusetts 02115

Find articles by Da Silva, W. in: PubMed | Google Scholar

Shriners Burns Institute and Department of Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio 45219

laboratories of the Research Division of St. Vincent Charity Hospital, Cleveland, Ohio

Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106

Department of Pediatrics, Harvard Medical School and Children's Hospital, Boston, Massachusetts 02115

Find articles by Rosen, F. in: PubMed | Google Scholar

Published April 1, 1969 - More info

Published in Volume 48, Issue 4 on April 1, 1969
J Clin Invest. 1969;48(4):642–653. https://doi.org/10.1172/JCI106022.
© 1969 The American Society for Clinical Investigation
Published April 1, 1969 - Version history
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Abstract

Plasma from persons with hereditary angioneurotic edema readily developed the capacity to increase vascular permeability and to induce the isolated rat uterus to contract. Both activities resided in a small, heat-stable molecule that was apparently a polypeptide. Crude preparations of the polypeptide were inactivated during incubation with trypsin. They also failed to produce pain and erythema, but caused markedly increased vascular permeability in human skin. These characteristics differ from those of bradykinin, from which crude preparations of the polypeptide could also be distinguished by electrophoretic mobility and paper chromatographic behavior. Proof that the polypeptide is truly different from bradykinin must await its further purification. Histamine played no role in the activities observed.

Although the enzymes functioning to release the permeability factor and kinin activities in hereditary angioneurotic edema plasma were not clearly defined, one or more plasma enzymes other than C′1 esterase presumably participated either in conjunction with C′1 esterase or in pari passu events to release the polypeptide mediating these activities.

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