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Free access | 10.1172/JCI105991

Studies on the purification of antihemophilic factor (factor VIII): I. Precipitation Of Antihemophilic Factor By Concanavalin A

Lawrence Kass, Oscar D. Ratnoff, and Myron A. Leon

Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106

Department of Microbiology, Case Western Reserve University, Cleveland, Ohio 44106

Department of Medicine, University Hospitals of Cleveland, Cleveland, Ohio 44106

Pathology Research Department, St. Luke's Hospital, Cleveland, Ohio 44106

Find articles by Kass, L. in: PubMed | Google Scholar

Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106

Department of Microbiology, Case Western Reserve University, Cleveland, Ohio 44106

Department of Medicine, University Hospitals of Cleveland, Cleveland, Ohio 44106

Pathology Research Department, St. Luke's Hospital, Cleveland, Ohio 44106

Find articles by Ratnoff, O. in: PubMed | Google Scholar

Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106

Department of Microbiology, Case Western Reserve University, Cleveland, Ohio 44106

Department of Medicine, University Hospitals of Cleveland, Cleveland, Ohio 44106

Pathology Research Department, St. Luke's Hospital, Cleveland, Ohio 44106

Find articles by Leon, M. in: PubMed | Google Scholar

Published February 1, 1969 - More info

Published in Volume 48, Issue 2 on February 1, 1969
J Clin Invest. 1969;48(2):351–358. https://doi.org/10.1172/JCI105991.
© 1969 The American Society for Clinical Investigation
Published February 1, 1969 - Version history
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Abstract

Concanavalin A precipitates antihemophilic factor from normal plasma. Combining this precipitation with other techniques, we were able to separate fractions rich in antihemophilic activity from human plasma with rapidity. The molecular weight of antihemophilic factor was estimated to be greater than 2 million. Presumably, antihemophilic factor is a large glycoprotein.

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