Citations to this article


The identification of human inflammatory cells that express inducible nitric oxide synthase and the clarification of the role of inducible nitric oxide synthase in human infectious or inflammatory processes have been elusive. In neutrophil-enriched fractions from urine, we demonstrate a 43-fold increase in nitric oxide synthase activity in patients with urinary tract infections compared with that in neutrophil-enriched fractions from noninfected controls. Partially purified inducible nitric oxide synthase is primarily membrane associated, calcium independent, and inhibited by arginine analogues with a rank order consistent with that of purified human inducible nitric oxide synthase. Molecular, biochemical, and immunocytochemical evidence unequivocally identifies inducible nitric oxide synthase as the major nitric oxide synthase isoform found in neutrophils isolated from urine during urinary tract infections. Elevated inducible nitric oxide synthase activity and elevated nitric oxide synthase protein measured in patients with urinary tract infections and treated with antibiotics does not decrease until 6-10 d of antibiotic treatment. The extended elevation of neutrophil inducible nitric oxide synthase during urinary tract infections may have both antimicrobial and proinflammatory functions.


M A Wheeler, S D Smith, G García-Cardeña, C F Nathan, R M Weiss, W C Sessa


Total citations by year

Year: 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 1998 1997 Total
Citations: 4 4 12 5 8 3 5 6 11 10 4 5 11 14 15 14 14 6 151
Citation information

Citations to this article in year 2000 (15)

Title and authors Publication Year
Neutrophils: Molecules, Functions and Pathophysiological Aspects
V Witko-Sarsat, P Rieu, B Descamps-Latscha, P Lesavre, L Halbwachs-Mecarelli
Laboratory Investigation 2000
Temporal changes of cytokines and nitric oxide products in urine from renal transplant patients
SD Smith, MA Wheeler, MI Lorber, RM Weiss
Kidney International 2000
Interaction between lung surfactant and nitric oxide production by alveolar macrophages stimulated by group B streptococci
RK Bouhafs, C Jarstrand
Pediatric Pulmonology 2000
New directions in allergic diseases: Mechanism-based anti-inflammatory therapies
PJ Barnes
Journal of Allergy and Clinical Immunology 2000
Compartmentalised inducible nitric-oxide synthase activity in septic shock
D Annane, S Sanquer, V Sébille, A Faye, D Djuranovic, JC Raphaël, P Gajdos, E Bellissant
The Lancet 2000
Reactive oxygen and nitrogen intermediates in the relationship between mammalian hosts and microbial pathogens
C Nathan, MU Shiloh
Proceedings of the National Academy of Sciences 2000
A neutrophil-derived anti-infective molecule: bactericidal/permeability-increasing protein
O Levy
Antimicrobial agents and chemotherapy 2000
Enhancement of neonatal innate defense: effects of adding an N-terminal recombinant fragment of bactericidal/permeability-increasing protein on growth and tumor necrosis factor-inducing activity of gram-negative bacteria tested in neonatal cord blood ex vivo
O Levy, RB Sisson, J Kenyon, E Eichenwald, AB Macone, D Goldmann
Infection and immunity 2000
Activation of nuclear factor kappaB and induction of inducible nitric oxide synthase by Ureaplasma urealyticum in macrophages
YH Li, ZQ Yan, JS Jensen, K Tullus, A Brauner
Infection and immunity 2000
Nitrite generation and antioxidant effects during neutrophil apoptosis
NL Misso, CD Peacock, DN Watkins, PJ Thompson
Free radical biology & medicine 2000
Nitric Oxide Regulates the Aggregation of Stimulated Human Neutrophils
T Forslund, HM Nilsson, T Sundqvist
Biochemical and Biophysical Research Communications 2000
Modulation of Polymorphonuclear Leukocytes Function by Nitric Oxide
S Sethi, M Dikshit
Thrombosis Research 2000
Nitric Oxide
JE Albina, JS Reichner
Nitric Oxide 2000
Reactive oxygen and nitrogen intermediates in the relationship between mammalian hosts and microbial pathogens
C Nathan, MU Shiloh
Proceedings of the National Academy of Sciences 2000
New treatments for asthma
PJ Barnes
European Journal of Internal Medicine 2000