Identification of the human T cell lymphoma virus in B cell lines established from patients with adult T cell leukemia.

D L Mann; J Clark; M Clarke; M Reitz; M Popovic; G Franchini; C D Trainor; D M Strong; W A Blattner; R C Gallo

doi:10.1172/JCI111418

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Published in Volume 74, Issue 1 on July 1, 1984
J Clin Invest. 1984;74(1):56–62. https://doi.org/10.1172/JCI111418.
© 1984 The American Society for Clinical Investigation

Published July 1, 1984 - Version history

Cell lines were established from the peripheral blood of two patients with adult T cell leukemia. In contrast to our previous experience, where all such lines expressed T cell markers, these two cell lines expressed B cell antigens and Ig light chains (kappa on CF-2, lambda on HS). Human T cell lymphoma proviral (HTLV) sequences were demonstrated in both cell lines. Since only a portion of the cells in culture expressed Ig light chains, experiments were carried out to exclude the possibility that the cultures were not a mixture of B and T or non-B cells. Cells that expressed kappa- or lambda-light chains were separated by cell sorting from kappa- or lambda-negative cells and replaced in culture. Light chain negative cells reexpressed light chains after time in culture. After 5-azacytidine treatment of the cell lines, all cells expressed Ig light chains. These studies show that the human retrovirus HTLV, which has been demonstrated to be associated with certain T cell malignancies, can infect B cells or B cell precursors.

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