Radioimmunoassay of Human Apolipoprotein CII: A STUDY IN NORMAL AND HYPERTRIGLYCERIDEMIC SUBJECTS

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Abstract

A specific, precise, and sensitive double-antibody radioimmunoassay for the measurement of human apolipoprotein CII (apoCII) was developed. ApoCII was labeled with 125I (chloramine-T) and monospecific antibody was raised in rabbits. No appreciable cross-reactivity with apolipoproteins CI, CIII, AI, AII, low density lipoproteins, and lipoprotein-free plasma was observed. Lipoproteins containing apoCII displaced the standard curve in parallel. ApoCII measurement was not affected by pretreatment of plasma with tetramethylurea, ethanol-diethyl ether, or heating.

Authors

M. L. Kashyap, L. S. Srivastava, C. Y. Chen, G. Perisutti, M. Campbell, R. F. Lutmer, C. J. Glueck