JCI - Upregulation of heme oxygenase-1 protects genetically fat Zucker rat livers from ischemia/reperfusion injury

Upregulation of heme oxygenase-1 protects genetically fat Zucker rat livers from ischemia/reperfusion injury

Farin Amersi, Roland Buelow, Hirohisa Kato, Bibo Ke, Ana J. Coito, Xiu-Da Shen, Delai Zhao, Joseph Zaky, Judy Melinek, Charles R. Lassman, Jay K. Kolls, J. Alam, Thomas Ritter, Hans-Dieter Volk, Douglas G. Farmer, Rafik M. Ghobrial, Ronald W. Busuttil, Jerzy W. Kupiec-Weglinski

Published in Volume 104, Issue 11

J Clin Invest. 1999; 104(11):1631–1639 doi:10.1172/JCI7903

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Figure 9

Western blot analysis of HO-1 protein expression in fatty livers with or without CoPP pretreatment. Livers were stored for 4 hours at 4°C, transplanted into lean Zucker recipients, and then harvested at 1, 7, 14, and 100 days. The expression of HO-1 was detected by a polyclonal rabbit anti-rat HO-1 Ab. Lane 1, CoPP day 1; lane 2, CoPP day 7; lane 3, CoPP day 14; lane 4, CoPP day 100; lane 5, untreated day 1; lane 6, untreated day 7; lane 7, untreated day 14; lane 8, untreated day 100. HO-1 migrates as a 32-kDa protein. The relative HO-1 expression levels analyzed by densitometer were (in absorbance units): 1.21, 1.27, 1.63, and 2.14 for CoPP-treated livers in lanes 1, 2, 3, and 4, respectively. The HO-1 expression for untreated controls in lanes 5, 6, 7, and 8 were (in absorbance units): 0.09, 0.17, 0.85, and 0.75, respectively. Data shown are representative of 3 independent experiments.