Tatiana Takiishi, Hannelie Korf, Tom L. Van Belle, Sofie Robert, Fabio A. Grieco, Silvia Caluwaerts, Letizia Galleri, Isabella Spagnuolo, Lothar Steidler, Karolien Van Huynegem, Pieter Demetter, Clive Wasserfall, Mark A. Atkinson, Francesco Dotta, Pieter Rottiers, Conny Gysemans, Chantal Mathieu
Local accumulation and autoAg-specific suppression by Tregs upon CT.
(A) Real-time PCR analysis of indicated mRNA isolated from CD4+CD25+ T cells sorted from spleen and PLN of long-standing normoglycemic, diabetic, and CT-cured NOD mice. (B) Number of Foxp3-expressing cells in or around the pancreatic islets as determined by manual counting of Foxp3+ cells on immunostained cryosections as shown in Supplemental Figure 9. (C) Representative microphotographs of immunostaining for Ki-67 and Foxp3 and nuclear staining using DAPI on paraffin sections of the pancreas of CT-cured NOD mice. White arrows indicate Ki-67+Foxp3+ double-positive cells, indicating proliferating Foxp3+ Tregs. Original magnification, ×63; detail, ×252. Statistical significance was calculated using Mann-Whitney U test (*P < 0.05, **P < 0.01, ***P < 0.001).