J Clin Invest.
Local accumulation and autoAg-specific suppression by Tregs upon CT.
(A) Real-time PCR analysis of indicated mRNA isolated from CD4+CD25+ T cells sorted from spleen and PLN of long-standing normoglycemic, diabetic, and CT-cured NOD mice. (B) Number of Foxp3-expressing cells in or around the pancreatic islets as determined by manual counting of Foxp3+ cells on immunostained cryosections as shown in Supplemental Figure 9. (C) Representative microphotographs of immunostaining for Ki-67 and Foxp3 and nuclear staining using DAPI on paraffin sections of the pancreas of CT-cured NOD mice. White arrows indicate Ki-67+Foxp3+ double-positive cells, indicating proliferating Foxp3+ Tregs. Original magnification, ×63; detail, ×252. Statistical significance was calculated using Mann-Whitney U test (*P < 0.05, **P < 0.01, ***P < 0.001).