Citations to this article


Tregs play an important role in protecting the skin from autoimmune attack. However, the extent of Treg trafficking between the skin and draining lymph nodes (DLNs) is unknown. We set out to investigate this using mice engineered to express the photoconvertible fluorescence protein Kaede, which changes from green to red when exposed to violet light. By exposing the skin of Kaede-transgenic mice to violet light, we were able to label T cells in the periphery under physiological conditions with Kaede-red and demonstrated that both memory phenotype CD4+Foxp3– non-Tregs and CD4+Foxp3+ Tregs migrated from the skin to DLNs in the steady state. During cutaneous immune responses, Tregs constituted the major emigrants and inhibited immune responses more robustly than did LN-resident Tregs. We consistently observed that cutaneous immune responses were prolonged by depletion of endogenous Tregs in vivo. In addition, the circulating Tregs specifically included activated CD25hi Tregs that demonstrated a strong inhibitory function. Together, our results suggest that Tregs in circulation infiltrate the periphery, traffic to DLNs, and then recirculate back to the skin, contributing to the downregulation of cutaneous immune responses.


Michio Tomura ... Osami Kanagawa, Kenji Kabashima


Total citations by year

Year: 2015 2014 2013 2012 2011 2010 Total
Citations: 9 21 16 22 13 6 87
Citation information

Citations to this article (87)

Title and authors Publication Year
The role of T and B cells in human atherosclerosis and atherothrombosis: Role of B and T cells in human atherosclerosis
E Ammirati, F Moroni, M Magnoni, PG Camici
Clinical & Experimental Immunology 2015
Population dynamics of islet-infiltrating cells in autoimmune diabetes
AM Magnuson, GM Thurber, RH Kohler, R Weissleder, D Mathis, C Benoist
Proceedings of the National Academy of Sciences 2015
Intestinal mucosal tolerance and impact of gut microbiota to mucosal tolerance
DA Chistiakov, YV Bobryshev, E Kozarov, IA Sobenin, AN Orekhov
Frontiers in microbiology 2015
Alteration in regulatory T cells and programmed cell death 1-expressing regulatory T cells in active generalized vitiligo and their clinical correlation
MK Tembhre, AS Parihar, VK Sharma, A Sharma, P Chattopadhyay, S Gupta
The British journal of dermatology 2015
Depletion of regulatory T cells in a hapten-induced inflammation model results in prolonged and increased inflammation driven by T cells: The role of regulatory T cells in CHS
AD Christensen, S Skov, PH Kvist, C Haase
Clinical & Experimental Immunology 2015
Vitamin D Actions on CD4+ T Cells in Autoimmune Disease
CE Hayes, SL Hubler, JR Moore, LE Barta, CE Praska, FE Nashold
Frontiers in immunology 2015
Cellular imaging in rheumatic diseases
RA Benson, IB McInnes, JM Brewer, P Garside
Nature Reviews Rheumatology 2015
Histamine suppresses regulatory T cells mediated by TGF- β in murine chronic allergic contact dermatitis
K Tamaka, M Seike, T Hagiwara, A Sato, H Ohtsu
Experimental Dermatology 2015
CCCTC-binding factor controls the homeostatic maintenance and migration of Langerhans cells
TG Kim, M Kim, JJ Lee, SH Kim, JH Je, Y Lee, MJ Song, Y Choi, YW Chung, CG Park, JW Cho, MG Lee, YS Lee, HP Kim
Journal of Allergy and Clinical Immunology 2015
Inflammatory lymphangiogenesis: cellular mediators and functional implications
KW Tan, SZ Chong, V Angeli
Angiogenesis 2014
Endoscopic photoconversion reveals unexpectedly broad leukocyte trafficking to and from the gut
AM Morton, E Sefik, R Upadhyay, R Weissleder, C Benoist, D Mathis
Proceedings of the National Academy of Sciences 2014
Chronic Ethanol Feeding Induces Subset Loss and Hyporesponsiveness in Skin T Cells
CP Parlet, TJ Waldschmidt, AJ Schlueter
Alcoholism Clinical and Experimental Research 2014
The regulatory T cells induction by epicutaneous immunotherapy is sustained and mediates long-term protection from eosinophilic disorders in peanut-sensitized mice
V Dioszeghy, L Mondoulet, V Dhelft, M Ligouis, E Puteaux, C Dupont, PH Benhamou
Clinical & Experimental Allergy 2014
Biological effects of space radiation and development of effective countermeasures
AR Kennedy
Life Sciences in Space Research 2014
Intravital Multiphoton Imaging of Cutaneous Immune Responses
K Kabashima, G Egawa
Journal of Investigative Dermatology 2014
Natural Killer T Cells Are Essential for the Development of Contact Hypersensitivity in BALB/c Mice
C Shimizuhira, A Otsuka, T Honda, A Kitoh, G Egawa, S Nakajima, C Nakashima, H Watarai, Y Miyachi, K Kabashima
Journal of Investigative Dermatology 2014
Hapten-Induced Contact Hypersensitivity, Autoimmune Reactions, and Tumor Regression: Plausibility of Mediating Antitumor Immunity
DA Erkes, SR Selvan
Journal of Immunology Research 2014
Ionizing radiation selectively reduces skin regulatory T cells and alters immune function
Y Zhou, H Ni, K Balint, JK Sanzari, T Dentchev, ES Diffenderfer, JM Wilson, KA Cengel, D Weissman
PloS one 2014
Tracking and quantification of dendritic cell migration and antigen trafficking between the skin and lymph nodes
M Tomura, A Hata, S Matsuoka, FH Shand, Y Nakanishi, R Ikebuchi, S Ueha, H Tsutsui, K Inaba, K Matsushima, A Miyawaki, K Kabashima, T Watanabe, O Kanagawa
Scientific Reports 2014
Molecular Immunotoxicology: Corsini/Molecular Immunotoxicology
SF Martin, PR Esser
Molecular Immunotoxicology: Corsini/Molecular Immunotoxicology 2014
New findings in allergic contact dermatitis:
N Fyhrquist, E Lehto, A Lauerma
Current Opinion in Allergy and Clinical Immunology 2014
The panoply of αβT cells in the skin
T Nomura, K Kabashima, Y Miyachi
Journal of Dermatological Science 2014
Commensal bacteria and cutaneous immunity
S Nakamizo, G Egawa, T Honda, S Nakajima, Y Belkaid, K Kabashima
Seminars in Immunopathology 2014
Perivascular leukocyte clusters are essential for efficient activation of effector T cells in the skin
Y Natsuaki, G Egawa, S Nakamizo, S Ono, S Hanakawa, T Okada, N Kusuba, A Otsuka, A Kitoh, T Honda, S Nakajima, S Tsuchiya, Y Sugimoto, KJ Ishii, H Tsutsui, H Yagita, Y Iwakura, M Kubo, L Ng, T Hashimoto, J Fuentes, E Guttman-Yassky, Y Miyachi, K Kabashima
Nature Immunology 2014
Dermal Vγ4+ γδ T Cells Possess a Migratory Potency to the Draining Lymph Nodes and Modulate CD8+ T-Cell Activity through TNF-α Production
S Nakamizo, G Egawa, M Tomura, S Sakai, S Tsuchiya, A Kitoh, T Honda, A Otsuka, S Nakajima, T Dainichi, H Tanizaki, M Mitsuyama, Y Sugimoto, K Kawai, Y Yoshikai, Y Miyachi, K Kabashima
Journal of Investigative Dermatology 2014