STAT3 and STAT1 mediate IL-11–dependent and inflammation-associated gastric tumorigenesis in gp130 receptor mutant mice
J. Clin. Invest. Matthias Ernst, et al. 118:1727 doi:10.1172/JCI34944 [
Go to this article.]
Figure 1Increased expression of IL-11 and other gp130 family cytokines in gastric tumors of
gp130Y757F/Y757F mice.
(
A) Quantitative RT-PCR (Q-PCR) analyses of
Il11,
Il6, and
Lif gene expression were performed on cDNA derived from total RNA prepared from antral gastric tissue of 14-week-old
gp130+/+ wild-type (+/+) and
gp130Y757F/Y757F (F/F) mice. Expression data from 3–5 samples per genotype following normalization for
18S expression are shown and are presented from replicate analysis as the mean fold induction ± SD relative to expression observed in
gp130+/+ samples. (
B) Immunoblot analyses were performed on lysates prepared from antral gastric tissue of
gp130+/+ and
gp130Y757F/Y757F mice using the indicated antibodies. Each lane represents tissue from an individual mouse. Densitometric quantitation of IL-11 in each of 3 representative samples per genotype was performed and normalized against ERK1/2 protein levels. Data are presented as the mean fold induction ± SD relative to expression in
gp130+/+ samples. (
C) Q-PCR gene expression analyses of
Il6ra,
Il11ra1, and
gp130 as in
A. *
P < 0.05 versus expression in
gp130+/+ samples.
