JCI - Low-field paramagnetic resonance imaging of tumor oxygenation and glycolytic activity in mice

Low-field paramagnetic resonance imaging of tumor oxygenation and glycolytic activity in mice

Shingo Matsumoto, Fuminori Hyodo, Sankaran Subramanian, Nallathamby Devasahayam, Jeeva Munasinghe, Emi Hyodo, Chandramouli Gadisetti, John A. Cook, James B. Mitchell, Murali C. Krishna

Published in Volume 118, Issue 5

J Clin Invest. 2008; 118(5):1965–1973 doi:10.1172/JCI34928

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Figure 3

In vivo oxygen mapping of SCC tumor-bearing and normal mouse legs, and visualization of the effect of carbogen breathing on tumor pO₂.

(A) Cartoon showing the positioning of the tumor-bearing and normal legs along with the fiducials in the resonator for combined EPRI and MRI. (B) T₂-weighted anatomical images (axial and coronal), in which tumor region could be clearly discriminated. Bright small circles are positional markers (fiducials) for coregistration of EPRI and MRI. (C) pO₂ images (axial and coronal) when breathing medical air. (D) pO₂ images (axial and coronal) 30 minutes after changing to breathing carbogen. (E) Histograms of pO₂ in the tumor region of the same mouse breathing medical air (blue) and carbogen (red). A net increase in the median pO₂ was noted upon carbogen breathing. (F) Blood volume image of coronal slice, in which a large blood vessel running vertically can be seen in the center of the tumor. Blood volume was calculated from a difference in image intensity before and after USPIO injection, therefore image intensity from positional markers was canceled. White lines indicate ROI.