Low-field paramagnetic resonance imaging of tumor oxygenation and glycolytic activity in mice
J. Clin. Invest. Shingo Matsumoto, et al. 118:1965 doi:10.1172/JCI34928 [Go to this article.]

Figure 3
In vivo oxygen mapping of SCC tumor-bearing and normal mouse legs, and visualization of the effect of carbogen breathing on tumor pO₂. (A) Cartoon showing the positioning of the tumor-bearing and normal legs along with the fiducials in the resonator for combined EPRI and MRI. (B) T₂-weighted anatomical images (axial and coronal), in which tumor region could be clearly discriminated. Bright small circles are positional markers (fiducials) for coregistration of EPRI and MRI. (C) pO₂ images (axial and coronal) when breathing medical air. (D) pO₂ images (axial and coronal) 30 minutes after changing to breathing carbogen. (E) Histograms of pO₂ in the tumor region of the same mouse breathing medical air (blue) and carbogen (red). A net increase in the median pO₂ was noted upon carbogen breathing. (F) Blood volume image of coronal slice, in which a large blood vessel running vertically can be seen in the center of the tumor. Blood volume was calculated from a difference in image intensity before and after USPIO injection, therefore image intensity from positional markers was canceled. White lines indicate ROI.