Antiestrogens reverse the adverse effects of E2 on ASL volume homeostasis.
(A) Mean change in fura-2 emission ratio in JME cells exposed to ATP (10 μM) and vehicle or ATP and either 10 nM E2, E2 and 1 μM ICI182780, ICI182780, E2 with 10 μM TXN, and ICI182780, or 10 μM TXN. All n = 6. (B and C) ASL height measured by XZ confocal microscopy in non-CF and CF HBECs, respectively. 200 μM mucosal ATP addition (squares); 200 μM mucosal ATP and 200 μM mucosal TXN (circles); 200 μM mucosal ATP and 200 μM mucosal TXN and 10 nM serosal E2 (triangles). (D) ASL height over time in non-CF (closed symbols) and CF (open symbols) HBECs with TXN ± bumetanide. Squares, 200 μM mucosal TXN; triangles, 200 μM mucosal TXN and 10 μM serosal bumetanide. All data shown as mean ± SEM. *P < 0.05 compared with ATP or TXN alone. †P < 0.05 versus 0 minutes.