Renin inhibition reduces hypercholesterolemia-induced atherosclerosis in mice
J. Clin. Invest. Hong Lu, et al. 118:984 doi:10.1172/JCI32970 [Go to this article.]

Figure 5
Renin deficiency in bone marrow–derived cells decreased atherosclerosis in Ldlr^–/– recipient mice fed a high-fat diet. (A) PCR confirmed the presence of the disrupted renin gene in bone marrow cells from recipient mice. PCR on bone marrow–derived DNA yielded an amplicon of 265 bp for the wild-type renin allele and 386 bp for the disrupted allele. The lanes were run on the same gel but were noncontiguous. (B) Atherosclerotic lesion size was measured on aortic arch intimal surfaces. Individual Ldlr^–/– recipients are represented by open symbols. Means of each group are represented by circles; error bars denote SEM. *P < 0.001 renin^+/+ donor (n = 12) versus renin^–/– donor (n = 10). (C) Renin deficiency in bone marrow–derived cells ablated renin expression in macrophages localized to atherosclerotic lesions in the aortic root. Immunostaining was performed in the atherosclerotic lesions of aortic root using rabbit anti-mouse macrophage antisera and a chicken anti-mouse renin IgY. Original magnification, ×200.