Acute effects of leptin require PI3K signaling in hypothalamic proopiomelanocortin neurons in mice
J. Clin. Invest. Jennifer W. Hill, et al. 118:1796 doi:10.1172/JCI32964 [Go to this article.]

Figure 2
Leptin causes depolarization of POMC neurons via a PI3K-dependent mechanism. (A) Current-clamp recordings demonstrated that disruption of PI3K signaling occluded the leptin-induced depolarization of POMC neurons from rest. Top: Characteristic leptin-induced (100 nM) depolarization of WT neurons. Middle: Absence of leptin-induced depolarization in POMC neurons from Pik3r1 POMCKO Pik3r2^–/– mice. Bottom: Leptin failed to depolarize POMC neurons in the presence of the PI3K inhibitor LY294002 (10 μM). (B) Leptin-induced responses of identified POMC neurons from WT, Pik3r1 POMCKO, and Pik3r1 POMCKO Pik3r2^–/– mice. P values compared with WT are shown.