Curcumin prevents and reverses murine cardiac hypertrophy
J. Clin. Invest. Hong-Liang Li, et al. 118:879 doi:10.1172/JCI32865 [Go to this article.]

Figure 4
Pretreatment with curcumin blocks p300-HAT activity. (A and B) The dose and time course of PE on HAT activity of p300. Cells were treated with different doses of PE (A) or with 100 μM PE for the indicated times (B) and then harvested and subjected to analysis of HAT activity as described in Methods. (C and D) Curcumin inhibited PE-induced p300-HAT activity. Cells were either pretreated for 60 minutes with different doses of curcumin and then incubated with 100 μM PE for 6 hours (C) or pretreated for 60 minutes with 25 μM curcumin and then incubated with 100 μM PE for different times up to 48 hours (D). (E and F) Effect of p300 on histone acetylation. Cells were infected with Ad-p300, Ad-DN-p300, or Ad-GFP for 24 hours and then treated with 100 μM PE for 6 hours. The global acetylation of histones (E) and the acetylation of histone H3, histone H4, and tubulin (F) were determined. Each assay was performed at least 3 times. *P < 0.05 versus control.