Csf3r mutations in mice confer a strong clonal HSC advantage via activation of Stat5
J. Clin. Invest. Fulu Liu, et al. 118:946 doi:10.1172/JCI32704 [Go to this article.]

Figure 1
The d715 G-CSFR confers a competitive repopulation advantage that is dependent upon exogenous G-CSF administration. Irradiated syngeneic mice were reconstituted with a 1:1 ratio of d715 G-CSFR (Ly5.2⁺) and wild-type (Ly5.1⁺) bone marrow cells. (A) The percentage of blood neutrophils (Gr-1), B lymphocytes (B220), T lymphocytes (CD3), and monocytes (F4/80) that were derived from d715 G-CSFR (d715) cells is shown (n = 9). (B and C) The mice were then treated with G-CSF (10 μg/kg/d; n = 5) or saline alone (n = 4) for 21 days and the contributions of d715 G-CSFR cells in the blood (B) and bone marrow (C) were measured. Data represent the mean ± SEM. *P < 0.05.