OX40 ligand expressed by DCs costimulates NKT and CD4⁺ Th cell antitumor immunity in mice
J. Clin. Invest. Jamal Zaini, et al. 117:3330 doi:10.1172/JCI32693 [Go to this article.]

Figure 1
Mice immunized with OVA-pulsed DCs in an OX40L-dependent manner. (A) Tumor growth in TNF-α–stimulated DCs. Mice were immunized with TNF-α–stimulated DCs pulsed with OVA (filled squares), DCs pulsed with OVA alone (circles), or TNF-α–stimulated DCs alone (triangles), and were challenged with EG7-OVA cells (day 0). Mice without any immunization (open squares) were used as controls. (B) OVA-specific cytotoxicity in TNF-α–stimulated DCs. Eight days after the immunization described in A, splenocytes were isolated and assayed for cytolytic function by using EG7-OVA or EL4 cells as target cells. (C and D) Flow cytometric analysis of TNF-α–stimulated DCs. DCs from wild-type or OX40L^–/– mice were stimulated with TNF-α and analyzed 2 days later for OX40L (C) or CD80 expression (D). Overlay (filled) histograms depict naive DCs. The percentages of stained cells above isotype control staining are shown in each panel. (E) Role of OX40L on DCs and in tumor growth. TNF-α–stimulated OX40L^–/– (circles) or wild-type DCs (filled squares) pulsed with OVA were used for the immunization challenge experiment. (F) Role of OX40L on DCs and OVA-specific cytotoxic T cells. Ten days after the immunization described in E, splenocytes were isolated and analyzed for OVA-reactive CD8⁺ T cells (CD8⁺H-2K^b/SIINFEKL pentamer⁺, boxed) by flow cytometry. The percentage of positive cells is listed. (G and H) Flow cytometric analysis of AdOX40L-modified DCs. DCs were transduced with AdOX40L or AdNull and analyzed 2 days later for OX40L (G) or CD80 expression (H). The percentages of stained cells above isotype control staining are shown in each panel. (I) Tumor growth in AdOX40L-modified DCs. AdOX40L- (circles) or AdNull-modified DCs (triangles) pulsed with OVA were used for the immunization challenge experiment. (J) OVA-specific cytotoxicity of AdOX40L-modified DCs. Eight days after the immunization described in I, splenocytes were assayed for cytolytic function.