Central insulin action regulates peripheral glucose and fat metabolism in mice
J. Clin. Invest. Linda Koch, et al. 118:2132 doi:10.1172/JCI31073 [Go to this article.]

Figure 5
Chronic intracerebroventricular infusion of insulin induces lipogenesis in C57BL/6 mice. (A) Epigonadal fat pad weight corrected for body weight of 11-week-old C57BL/6 mice receiving chronic intracerebroventricular infusion of carrier (dark purple bars, n = 5) or insulin solution (light purple bars, n = 5) at a rate of 200 μU/d over 7 days, and of 14-week-old Control^Δper (black bars, n = 19), IR^Δper (white bars, n = 15), Control^Δwb (dark gray bars, n = 13), and IR^Δwb mice (light gray bars, n = 16) mice 30 days after start of inducer administration. Values are mean ± SEM. *P ≤ 0.05; ***P ≤ 0.001. (B) H&E staining of WAT of 11-week-old C57BL/6 mice receiving a chronic intracerebroventricular infusion of carrier or insulin solution at a rate of 200 μU/d over 7 days. Magnification, ×100. (C) Mean adipocyte size of 11-week-old C57BL/6 mice receiving chronic intracerebroventricular infusion of carrier (dark purple bar, n = 3) or insulin solution (light purple bar, n = 3) at a rate of 200 μU/d over 7 days. Values are mean ± SEM. **P ≤ 0.01 versus control. (D) Relative mRNA expression of lipoprotein lipase (LPL) in WAT of 11-week-old C57BL/6 mice receiving chronic intracerebroventricular infusion of carrier (dark purple bar, n = 5) or insulin solution (light purple bar, n = 5) at a rate of 200 μU/d over 7 days. Values are mean ± SEM. *P ≤ 0.05 versus control.