Central insulin action regulates peripheral glucose and fat metabolism in mice
J. Clin. Invest. Linda Koch, et al. 118:2132 doi:10.1172/JCI31073 [Go to this article.]

Figure 2
IR expression and insulin-stimulated signaling in IR^Δper mice. (A) Western blot analysis of IR and AKT (loading control) in whole brain, hypothalamus, cortex, cerebellum, heart, skeletal muscle, liver, and pancreas of 14-week-old tamoxifen-treated IR^Δper and Control^Δper mice. Indicated tissues were dissected 25 days after the last administration of tamoxifen. Animal groups comprised a minimum of 7 IR^Δper and 9 Control^Δper mice, except for the western blot analysis in heart, which represents 5 IR^Δper and 4 Control^Δper mice. (B) Comparative densitometric analysis of IR expression of 14-week-old Control^Δper (black bars) and IR^Δper mice (white bars) of the western blot analysis shown in A. Values are mean ± SEM. *P ≤ 0.05; **P ≤ 0.01 versus control. (C) Western blot analysis of IR, pAKT, and AKT (loading control) in skeletal muscle and liver of 14-week-old Control^Δper and IR^Δper mice injected with either saline (–) or insulin (+) into the vena cava inferior to assess remaining insulin signaling after IR deletion. Animal groups included 8 IR^Δper and 8 Control^Δper mice. (D) Comparative densitometric analysis of pAKT of 14-week-old Control^Δper and IR^Δper mice of the western blot analysis shown in C. pAKT protein amount was normalized to AKT protein expression. pAKT of saline-injected mice was set to 100%. Values are mean ± SEM. *P ≤ 0.05, **P ≤ 0.01 versus control. Black bars, saline; white bars, insulin.