Autophagy inhibition enhances therapy-induced apoptosis in a Myc-induced model of lymphoma
J. Clin. Invest. 117:2 doi:10.1172/JCI28833 [Go to this article.]

Figure 5
Effects of p53 activation with and without knockdown of ATG5 on tumor cell death.

(A) Western blot against ATG5 of lysates from Myc/p53ER^TAM/vector cells (V), Myc/p53ER^TAM/HC cells (HC), Myc/p53ER^TAM/shATG5 h2 cells (h2), and Myc/p53ER^TAM/shATG5 h7 cells (h7). Actin was used as a loading control. (B) Activation of p53 in HC and shATG5 cells. HC, h2, and h7 lymphoma cells were treated with 200 nM hTAM daily. (C) Activation of p53 plus methyl pyruvate (MP). HC, h2, and h7 lymphoma cells were treated daily with hTAM plus 1 mM MP. (B and C) Viable cell number as determined by trypan blue exclusion was counted daily. Reported values are mean ± SD of triplicate samples from a representative experiment.