A cancer-specific transcriptional
signature in human neoplasia
J. Clin. Invest. Francesco Nicassio, et al. 115:3015 doi:10.1172/JCI24862 [
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Figure 1E1A-induced genes and their classification. (
A) The E1A-induced genes are indicated by their accession numbers (acc. no.) and short names. Q–RT-PCR analysis was performed under the following conditions: (a) overexpression of E1A (not shown here; see Supplemental Table 2); (b) overexpression of the E1A mutant YH47/dl928 (YH47); (c) overexpression of E2F1 (E2F); and (d) removal of
Rb (
Rb–/–). Values were normalized to those obtained upon overexpression of E1A and are expressed with a color code. YH47 and E2F conditions, red/blue, > or < 35% vs. the E1A-induced condition, respectively;
Rb–/– condition, red/blue, > or < 50% vs. the E1A-induced condition, respectively. Actual values are in Supplemental Table 4, and details on each experimental condition are in Supplemental Tables 5–7. Asterisks mark nonsignificant values (
P > 0.05). Early and late refer to activation by E1A (actual values are in Supplemental Table 3). (
B) Examples of data reported in
A. The bar graphs show transcript levels (measured by Q–RT-PCR) of representative genes, as indicated. E1A-early and -late, overexpression of E1A at early and late time points; mock, dl312 adenovirus control; YH47, overexpression of the E1A mutant YH47/dl928; E2F, overexpression of E2F1; MSC-E1A, overexpression of E1A in MSCs;
Rb–/–, removal of
Rb; MSC prolif., proliferating myoblasts. Values are normalized to mock-infected myotubes (assumed as 1.0). Results are from a single experiment; statistical analyses on repeated experiments are indicated in Supplemental Tables 2–7.
