The AML1-ETO fusion gene and the
FLT3 length mutation collaborate
in inducing acute leukemia in mice
J. Clin. Invest. Christina Schessl, et al. 115:2159 doi:10.1172/JCI24225 [
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Figure 6Analysis of proviral integrations. (
A) Southern blot analysis of genomic DNA from different primary mice and a secondary recipient to detect clonal proviral integrations. DNA was digested with
EcoRI, which cuts once in the proviral sequence, and blots were hybridized to a GFP/YFP probe. The mouse numbers are indicated (corresponding to those in Table
2). S, spleen; PB, peripheral blood; sec. of 21, second mouse of 1° mouse no. 21. (
B) Bubble PCR analyses of retroviral integration sites in diseased mice. The bands (A–I) were isolated, subcloned, and sequenced. A description of the PCR products is given in Table
3. Asterisk indicates resolution as 2 unique bands after subcloning and sequencing of integration sites.
