T cell–dependent production of IFN-γ by NK cells in response to influenza A virus
J. Clin. Invest. Xiao-Song He, et al. 114:1812 doi:10.1172/JCI22797 [Go to this article.]

Figure 5
IFN-γ response of NK cells to fluA required CD3⁺CD56^– cells but not CD3⁺CD56⁺ cells. (A) A blood sample was split into 2 fractions. CD3⁺ cells were depleted from fraction I. CD56⁺, CD36⁺, CD19⁺, and CD16⁺ cells were depleted from fraction II to yield enriched CD56^– T cells. Combination of fractions I and II resulted in a population depleted of CD3⁺CD56⁺ cells. (B) Flow-cytometric analysis of the unfractionated PBMCs, CD3-depleted PBMCs, CD56^– T cells, as well as CD3⁺CD56⁺ cell–depleted PBMCs. Displayed in the dot plots are cells gated on lymphocyte population by forward scattering and side scattering. (C) FluA-induced production of IFN-γ by NK cells in unfractionated PBMCs, CD3-depleted PBMCs, and CD3⁺CD56⁺ cell-depleted PBMCs. Cells were incubated with fluA for 17 hours. Displayed are cells gated on CD3^– lymphocyte population. The numbers in the dot plots are percentages of IFN-γ⁺ cells among CD3^–CD56⁺ NK cells. Similar results were obtained in experiments using blood samples from 2 other donors.