Clonally expanded novel multipotent stem cells from human bone marrow regenerate myocardium after myocardial infarction
J. Clin. Invest. Young-sup Yoon, et al. 115:326 doi:10.1172/JCI22326 [Go to this article.]

Figure 2
In vitro differentiation of hBMSCs into EC and SMC lineages. (A) Hoffman phase-contrast image (upper left panel) 5 days after culture with DMEM in gelatin-coated glass chambers shows that hBMSCs have formed typical vascular tubelike structures. Immunofluorescent imaging demonstrates that hBMSCs express EC-specific proteins such as vWFa, KDR, VE-cadherin, CD31, and ULEX after culturing in EC differentiation media for 14 days. (B) RT-PCR analysis using EC-specific primers VE-cadherin, CD34, KDR, Tie2, and CD31 also confirms the differentiation of hBMSCs into EC phenotypes. Lane 1, size marker; lane 2, before differentiation; lane 3, induced differentiation; lane 4, positive control. (C) hBMSCs cultured in 2% DMEM containing PDGF-BB for 14 days demonstrate the expression of SMC-specific proteins α-SMA and calponin by immunofluorescent staining. (D) RT-PCR analysis shows that SMC-specific genes PDGFR-β, α-SMA, SM22α, and SM1 are only expressed after induction of differentiation. Lane 1, size marker; lane 2, before differentiation; lane 3, induced differentiation; lane 4, positive control. The heavy band in the lanes of the size markers in B and D represents 600 bp. Scale bar: 100 μm.