Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell–driven B cell responses
J. Clin. Invest. Hyung W. Lim, et al. 114:1640 doi:10.1172/JCI22325 [Go to this article.]

Figure 7
CD4⁺CD25⁺CD69^– Tregs suppress GC-Th cell_induced Ig production by naive, GC, and memory B cells. (A) CD4⁺CD25⁺CD69^– Tregs and CD57⁺ GC-Th cells were cocultured with naive, GC, or memory B cells. CD4⁺CD25⁺CD69^– Tregs (10⁵) were added to the cultures of 10⁵ CD57⁺ GC-Th cells and 10⁵ B cells. *Less than 1% of the B plus GC-Th levels. (B) Various numbers of CD4⁺CD25⁺CD69^– Tregs (0.5 × 10⁵ to 2 × 10⁵) were cultured with CD57⁺ GC-Th cells and GC-B cells. (C) Neutralizing antibodies to CTLA-4 and TGF-β1 or isotype control antibodies were added to the cultures of CD57⁺ GC-Th cells, GC-B cells, and Tregs. Concentrations of IgM, IgG, IgA, and IgE were determined by ELISA after 5 days in culture. ELISA was performed in triplicate (A and C) or duplicate (B), and the averages and differences are shown. Representative data from 3 independent experiments are shown in A and B. For C, the data obtained from 3 independent experiments were combined, and averages and SEM are shown. *Significant differences from GC-B + GC-Th + Treg + isotype Ab culture. The P values were 0.0001 (a), 0.0001 (b), 0.0001 (c), 0.0001 (d), 0.001 (e), 0.006 (f), 0.01 (g), 0.03 (h), 0.004 (i), and 0.003 (j).