Platelet-derived lysophosphatidic acid supports the progression of osteolytic bone metastases in breast cancer
J. Clin. Invest. Ahmed Boucharaba, et al. 114:1714 doi:10.1172/JCI22123 [Go to this article.]

Figure 1
Expression of LPA receptors in breast cancer and mitogenic activity of LPA in breast cancer cell lines. (A) RT-PCR experiments using total RNA isolated from human primary tumors: fibroadenomas (F.Ad 1, F.Ad 2), ductal carcinomas (T 1, T 2). Expected size of amplification products for LPA₁ (1), LPA₂ (2), LPA₃ (3), and GAPDH (G) are 428, 352, 256, and 470 bp, respectively. (B) Human breast cancer cell lines were stimulated with increasing concentrations of LPA and pulsed with [³H]-thymidine. Cell proliferation was assessed after quantification of [³H]-thymidine incorporation. Data are expressed in cpm as the mean ± SD of 6 replicates and are representative of at least 3 separate experiments. Insets: RT-PCR amplification products for LPA₁, LPA₂, LPA₃, and GAPDH using total RNA isolated from each indicated cell line.