EVI1 induces myelodysplastic syndrome in mice
J. Clin. Invest. Silvia Buonamici, et al. 114:713 doi:10.1172/JCI21716 [Go to this article.]

Figure 1
EVI1 causes a fatal disease in reconstituted mice. (A) Retroviral DNA constructs used in Phoenix cell line. The 5′ long terminal repeat (LTR) provides the promoter for a transcript that includes EVI1 and a gene encoding resistance to G418 (NeoR, indicated by the arrow). Internal ribosome entry site (IRES) is required for the translation of the NeoR transcript. HIII is the unique HindIII restriction site present in the EVI1 cDNA. The location of the probe used for Southern blot analysis is indicated. HA, hemagglutinin epitope. (B) Western blot analysis of EVI1-producing packaging Phoenix cells (lane 1), vector-producing packaging Phoenix cells (lane 2), control mouse BM cells (lane 3), and EVI1-positive BM cells (lanes 4 and 5) confirms the appropriate expression of EVI1 only in EVI1-positive samples (lanes 1, 4, and 5). (C) Southern blot analysis of BM cells from reconstituted EVI1-positive mice (lanes 4 and 5) and control mice (lane 3). (D) Counts of wbc’s (diamonds, × 10³/μl), rbc’s (squares, × 10⁶/μl), platelets (horizontal bars, × 10⁵/μl), and levels of hemoglobin (crosses, g/dl) in PB of EVI1-positive mice (left) and in control mice (right). (E) The solid line shows the Kaplan-Meier survival curve of EVI1-positive reconstituted mice. All EVI1-positive mice died or were killed because of disease conditions. The dashed line represents the survival of the control mice.