Overexpression of Insig-1 in the livers of transgenic mice inhibits SREBP processing and reduces insulin-stimulated lipogenesis
J. Clin. Invest. Luke J. Engelking, et al. 113:1168 doi:10.1172/JCI20978 [Go to this article.]

Figure 3
Relative amount of various mRNAs in the livers of WT and TgInsig-1 mice fed with increasing amounts of cholesterol (0.02–0.5%). The mice used here are the same as those used in Figure 2 and Supplemental Table 1. Total RNA from four mouse livers was pooled and subjected to real-time PCR quantification as described in Methods. Each value represents the amount of mRNA relative to that in WT mice fed a chow diet (0.02% cholesterol), which is arbitrarily defined as 1. FAS, fatty acid synthase; FDP, farnesyl diphosphate; GPAT, glycerol-3-phosphate acyltransferase; SCD-1, stearoyl CoA desaturase-1.