JCI - Distal renal tubular acidosis in mice that lack the forkhead transcription factor Foxi1

Distal renal tubular acidosis in mice that lack the forkhead transcription factor Foxi1

Sandra Rodrigo Blomqvist, Hilmar Vidarsson, Sharyn Fitzgerald, Bengt R. Johansson, Anna Ollerstam, Russell Brown, A. Erik G. Persson, Göran Bergström, Sven Enerbäck

Published in Volume 113, Issue 11

J Clin Invest. 2004; 113(11):1560–1570 doi:10.1172/JCI20665

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Figure 2

The cRNA in situ hybridization of mouse kidney sections of WT and Foxi1^–/– origin using antisense probes for Foxi1 (A–D) and Pds (E–G). In cortical sections, Foxi1-positive cells are localized to tubular regions (A). At higher magnification, a scattered pattern of hybridization signals is clearly visualized. While some cells are densely stained, others remain unstained (B). In medulla sections, cells positive for Foxi1 are scattered and distributed along the collecting ducts (C). At a higher magnification, it is evident that some cells stain and others lack any clear hybridization signal (D). The antisense Pds cRNA probe identifies a pattern of scattered tubular cells in cortical sections (E–G). No signals from cells in the medulla can be identified (E). At higher magnification, it is clear that some cells stain, while others do not (F and G), a pattern very similar to that for Foxi1 in the cortex (A and B). No Pds signals can be identified in Foxi1^–/– kidneys. Scale bars: 40 ∝m.