Administration of CD34+ cells after stroke enhances neurogenesis via angiogenesisin a mouse model
J. Clin. Invest. Akihiko Taguchi, et al. 114:330
doi:10.1172/JCI20622 [Go to this article.]

Figure 5
Therapeutic neovascularization, due to CD34+ cell transplantation after stroke, enhances neurogenesis. (AF) On day 14 after CD34 cell transplantation, mature cortical neurons were observed up to the edge of the ischemic region displaying neuronal markers, NeuN (A) and MAP-2 (B), whereas only a thin layer of migrating PSA-NCAM+ NPCs was observed at the ischemic edge (C). In contrast, after transplantation of CD34+ cells, expanded cortical areas displaying a low density of NeuN+ (D) and MAP-2+ cells (E) were observed beyond the boundary demarcating mature neurons. Migration of NPCs into this expanded area was also observed by PSA-NCAM staining (F). (GI) On day 14, TUNEL+ cells were visualized around the lower part of the expanded cortical area. Whereas massive cell death was observed in animals receiving CD34 cell transplantation (G), the number of TUNEL+ profiles was strongly reduced in mice transplanted with CD34+ cells (H). (I) The average number of TUNEL+ cells per HPF. Three sections were evaluated in each animal; n = 5 per group. Arrowheads indicate the expanded cortical areas displaying a low density of indicated marker. Scale bars: 100 μm (A) and 50 μm (G). *P < 0.05 for CD34+ versus CD34 cell transplantation.