Gain-of-function mutation in the KCNMB1 potassium channel subunit is associated with low prevalence of diastolic hypertension
J. Clin. Invest. José M. Fernández-Fernández, et al. 113:1032 doi:10.1172/JCI20347 [Go to this article.]

Figure 3
Plots of conductance versus voltage for BK channel currents at different Ca²⁺ concentrations. BK channel currents were recorded from patches excised from cells expressing α (A), α+β_1WT (B), α+β_1E65K (C), and α+β_1WT+β_1E65K (D) channel subunits. Normalized conductance was obtained for each test potential from the peak tail current at –80 mV measured at 0 (circles), 100 nM (squares), 500 nM (triangles), and 10 μM (inverted triangles) Ca²⁺. Solid curves represent fits to the Boltzmann equation. The data at 0 and 100 nM Ca²⁺ were normalized using the value obtained at 500 nM as maximum tail current. To obtain an average G-V curve for each case, individual curves were displaced along the voltage axis by ΔV = (<V_1/2> – V_1/2) (where <V_1/2> denotes the average V_1/2 for each transfection group). The 300–360 resulting points were then reduced to 61 with the smoothing function of SigmaPlot (SPSS Inc., Richmond, California, USA).