The IL-12Rβ2 gene functions as a tumor suppressor in human B cell malignancies
J. Clin. Invest. Irma Airoldi, et al. 113:1651 doi:10.1172/JCI20303 [
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Figure 4Induction of IL-12Rβ2 by treatment with 5-Aza-2′-deoxycytidine in primary tumors. (
A) Left panel: Freshly isolated B-CLL cells (T0), B-CLL cells cultured with medium alone (medium) or in the presence of 5 ∝M 5-Aza-2′-deoxycytidine (Aza) for 48 to 96 hours. One sample representative of the four tested is shown. Right panel. Purified FL cells cultured with medium alone (medium) or in the presence of 5-Aza-2′-deoxycytidine for 72h. One sample representative of the two tested is shown. (
B) Apoptosis of primary neoplastic B cells incubated with 5-Aza-2′-deoxycytidine for 72 hours and subsequently cultured with hrIL-12 for an additional 48 hours, as assessed by the TUNEL assay. Histogram in the left panel shows the percentages of apoptotic cells detected following culture with medium alone (white bar); with 5-Aza-2′-deoxycytidine for 72 hours and subsequently with medium for additional 48 hours (gray bar); or with 5-Aza-2′-deoxycytidine for 72 hours and subsequently with hrIL-12 for an additional 48 hours (black bar, Aza + IL-12). Two B-CLL and 2 FL samples are shown. In the right panel, one representative experiment performed with B-CLL1 sample is shown. TUNEL positive nuclei stained with FITC are green, whereas interphase nuclei stained with DAPI are blue (magnification, ∞20).
