Mechanical strain exacerbates <i>Pseudomonas</i> infection in an organoid-based pneumonia-on-a-chip model

Karen Hoffmann; Ulrike Behrendt; Peter Pennitz; Holger Kirsten; Jessica Pohl; Elena Lopez-Rodriguez; Chantal Weissfuss; Jens Kollmeier; Mario Tönnies; Sebastian Brill; Konrad Steinestel; Martin Witzenrath; Werner Wenzel; Christian Zobel; Geraldine Nouailles

doi:10.1172/JCI192454

¹Department of Infectious Diseases, Respiratory Medicine and Critical Care, Charité - Universitätsmedizin Berlin, Berlin, Germany

²Institute for Medical Informatics, Statistics, and Epidemiology, Universität Leipzig, ScaDS.AI, Leipzig, Germany

³Department of Internal Medicine and Department of Microbiology and Hospital, Bundeswehrkrankenhaus Berlin, Berlin, Germany

⁴Institute of Functional Anatomy, Charité - Universitätsmedizin Berlin, Berlin, Germany

⁵Respiratory Diseases Clinic Heckeshorn, Helios Klinikum Emil von Behring GmbH, Berlin, Germany

⁶Department for Thoracic-Surgery and Institute of Pathology and Molecular Pa, Bundeswehrkrankenhaus Ulm, Ulm, Germany

Find articles by Hoffmann, K. in: PubMed | Google Scholar

¹Department of Infectious Diseases, Respiratory Medicine and Critical Care, Charité - Universitätsmedizin Berlin, Berlin, Germany

²Institute for Medical Informatics, Statistics, and Epidemiology, Universität Leipzig, ScaDS.AI, Leipzig, Germany

³Department of Internal Medicine and Department of Microbiology and Hospital, Bundeswehrkrankenhaus Berlin, Berlin, Germany

⁴Institute of Functional Anatomy, Charité - Universitätsmedizin Berlin, Berlin, Germany

⁵Respiratory Diseases Clinic Heckeshorn, Helios Klinikum Emil von Behring GmbH, Berlin, Germany

⁶Department for Thoracic-Surgery and Institute of Pathology and Molecular Pa, Bundeswehrkrankenhaus Ulm, Ulm, Germany

Find articles by Behrendt, U. in: PubMed | Google Scholar

¹Department of Infectious Diseases, Respiratory Medicine and Critical Care, Charité - Universitätsmedizin Berlin, Berlin, Germany

²Institute for Medical Informatics, Statistics, and Epidemiology, Universität Leipzig, ScaDS.AI, Leipzig, Germany

³Department of Internal Medicine and Department of Microbiology and Hospital, Bundeswehrkrankenhaus Berlin, Berlin, Germany

⁴Institute of Functional Anatomy, Charité - Universitätsmedizin Berlin, Berlin, Germany

⁵Respiratory Diseases Clinic Heckeshorn, Helios Klinikum Emil von Behring GmbH, Berlin, Germany

⁶Department for Thoracic-Surgery and Institute of Pathology and Molecular Pa, Bundeswehrkrankenhaus Ulm, Ulm, Germany

Find articles by Pennitz, P. in: PubMed | Google Scholar

¹Department of Infectious Diseases, Respiratory Medicine and Critical Care, Charité - Universitätsmedizin Berlin, Berlin, Germany

²Institute for Medical Informatics, Statistics, and Epidemiology, Universität Leipzig, ScaDS.AI, Leipzig, Germany

³Department of Internal Medicine and Department of Microbiology and Hospital, Bundeswehrkrankenhaus Berlin, Berlin, Germany

⁴Institute of Functional Anatomy, Charité - Universitätsmedizin Berlin, Berlin, Germany

⁵Respiratory Diseases Clinic Heckeshorn, Helios Klinikum Emil von Behring GmbH, Berlin, Germany

⁶Department for Thoracic-Surgery and Institute of Pathology and Molecular Pa, Bundeswehrkrankenhaus Ulm, Ulm, Germany

Find articles by Kirsten, H. in: PubMed | Google Scholar

¹Department of Infectious Diseases, Respiratory Medicine and Critical Care, Charité - Universitätsmedizin Berlin, Berlin, Germany

²Institute for Medical Informatics, Statistics, and Epidemiology, Universität Leipzig, ScaDS.AI, Leipzig, Germany

³Department of Internal Medicine and Department of Microbiology and Hospital, Bundeswehrkrankenhaus Berlin, Berlin, Germany

⁴Institute of Functional Anatomy, Charité - Universitätsmedizin Berlin, Berlin, Germany

⁵Respiratory Diseases Clinic Heckeshorn, Helios Klinikum Emil von Behring GmbH, Berlin, Germany

⁶Department for Thoracic-Surgery and Institute of Pathology and Molecular Pa, Bundeswehrkrankenhaus Ulm, Ulm, Germany

Find articles by Pohl, J. in: PubMed | Google Scholar

¹Department of Infectious Diseases, Respiratory Medicine and Critical Care, Charité - Universitätsmedizin Berlin, Berlin, Germany

²Institute for Medical Informatics, Statistics, and Epidemiology, Universität Leipzig, ScaDS.AI, Leipzig, Germany

³Department of Internal Medicine and Department of Microbiology and Hospital, Bundeswehrkrankenhaus Berlin, Berlin, Germany

⁴Institute of Functional Anatomy, Charité - Universitätsmedizin Berlin, Berlin, Germany

⁵Respiratory Diseases Clinic Heckeshorn, Helios Klinikum Emil von Behring GmbH, Berlin, Germany

⁶Department for Thoracic-Surgery and Institute of Pathology and Molecular Pa, Bundeswehrkrankenhaus Ulm, Ulm, Germany

Find articles by Lopez-Rodriguez, E. in: PubMed | Google Scholar |

¹Department of Infectious Diseases, Respiratory Medicine and Critical Care, Charité - Universitätsmedizin Berlin, Berlin, Germany

²Institute for Medical Informatics, Statistics, and Epidemiology, Universität Leipzig, ScaDS.AI, Leipzig, Germany

³Department of Internal Medicine and Department of Microbiology and Hospital, Bundeswehrkrankenhaus Berlin, Berlin, Germany

⁴Institute of Functional Anatomy, Charité - Universitätsmedizin Berlin, Berlin, Germany

⁵Respiratory Diseases Clinic Heckeshorn, Helios Klinikum Emil von Behring GmbH, Berlin, Germany

⁶Department for Thoracic-Surgery and Institute of Pathology and Molecular Pa, Bundeswehrkrankenhaus Ulm, Ulm, Germany

Find articles by Weissfuss, C. in: PubMed | Google Scholar

¹Department of Infectious Diseases, Respiratory Medicine and Critical Care, Charité - Universitätsmedizin Berlin, Berlin, Germany

²Institute for Medical Informatics, Statistics, and Epidemiology, Universität Leipzig, ScaDS.AI, Leipzig, Germany

³Department of Internal Medicine and Department of Microbiology and Hospital, Bundeswehrkrankenhaus Berlin, Berlin, Germany

⁴Institute of Functional Anatomy, Charité - Universitätsmedizin Berlin, Berlin, Germany

⁵Respiratory Diseases Clinic Heckeshorn, Helios Klinikum Emil von Behring GmbH, Berlin, Germany

⁶Department for Thoracic-Surgery and Institute of Pathology and Molecular Pa, Bundeswehrkrankenhaus Ulm, Ulm, Germany

Find articles by Kollmeier, J. in: PubMed | Google Scholar

¹Department of Infectious Diseases, Respiratory Medicine and Critical Care, Charité - Universitätsmedizin Berlin, Berlin, Germany

²Institute for Medical Informatics, Statistics, and Epidemiology, Universität Leipzig, ScaDS.AI, Leipzig, Germany

³Department of Internal Medicine and Department of Microbiology and Hospital, Bundeswehrkrankenhaus Berlin, Berlin, Germany

⁴Institute of Functional Anatomy, Charité - Universitätsmedizin Berlin, Berlin, Germany

⁵Respiratory Diseases Clinic Heckeshorn, Helios Klinikum Emil von Behring GmbH, Berlin, Germany

⁶Department for Thoracic-Surgery and Institute of Pathology and Molecular Pa, Bundeswehrkrankenhaus Ulm, Ulm, Germany

Find articles by Tönnies, M. in: PubMed | Google Scholar

¹Department of Infectious Diseases, Respiratory Medicine and Critical Care, Charité - Universitätsmedizin Berlin, Berlin, Germany

²Institute for Medical Informatics, Statistics, and Epidemiology, Universität Leipzig, ScaDS.AI, Leipzig, Germany

³Department of Internal Medicine and Department of Microbiology and Hospital, Bundeswehrkrankenhaus Berlin, Berlin, Germany

⁴Institute of Functional Anatomy, Charité - Universitätsmedizin Berlin, Berlin, Germany

⁵Respiratory Diseases Clinic Heckeshorn, Helios Klinikum Emil von Behring GmbH, Berlin, Germany

⁶Department for Thoracic-Surgery and Institute of Pathology and Molecular Pa, Bundeswehrkrankenhaus Ulm, Ulm, Germany

Find articles by Brill, S. in: PubMed | Google Scholar

¹Department of Infectious Diseases, Respiratory Medicine and Critical Care, Charité - Universitätsmedizin Berlin, Berlin, Germany

²Institute for Medical Informatics, Statistics, and Epidemiology, Universität Leipzig, ScaDS.AI, Leipzig, Germany

³Department of Internal Medicine and Department of Microbiology and Hospital, Bundeswehrkrankenhaus Berlin, Berlin, Germany

⁴Institute of Functional Anatomy, Charité - Universitätsmedizin Berlin, Berlin, Germany

⁵Respiratory Diseases Clinic Heckeshorn, Helios Klinikum Emil von Behring GmbH, Berlin, Germany

⁶Department for Thoracic-Surgery and Institute of Pathology and Molecular Pa, Bundeswehrkrankenhaus Ulm, Ulm, Germany

Find articles by Steinestel, K. in: PubMed | Google Scholar

¹Department of Infectious Diseases, Respiratory Medicine and Critical Care, Charité - Universitätsmedizin Berlin, Berlin, Germany

²Institute for Medical Informatics, Statistics, and Epidemiology, Universität Leipzig, ScaDS.AI, Leipzig, Germany

³Department of Internal Medicine and Department of Microbiology and Hospital, Bundeswehrkrankenhaus Berlin, Berlin, Germany

⁴Institute of Functional Anatomy, Charité - Universitätsmedizin Berlin, Berlin, Germany

⁵Respiratory Diseases Clinic Heckeshorn, Helios Klinikum Emil von Behring GmbH, Berlin, Germany

⁶Department for Thoracic-Surgery and Institute of Pathology and Molecular Pa, Bundeswehrkrankenhaus Ulm, Ulm, Germany

Find articles by Witzenrath, M. in: PubMed | Google Scholar |

¹Department of Infectious Diseases, Respiratory Medicine and Critical Care, Charité - Universitätsmedizin Berlin, Berlin, Germany

²Institute for Medical Informatics, Statistics, and Epidemiology, Universität Leipzig, ScaDS.AI, Leipzig, Germany

³Department of Internal Medicine and Department of Microbiology and Hospital, Bundeswehrkrankenhaus Berlin, Berlin, Germany

⁴Institute of Functional Anatomy, Charité - Universitätsmedizin Berlin, Berlin, Germany

⁵Respiratory Diseases Clinic Heckeshorn, Helios Klinikum Emil von Behring GmbH, Berlin, Germany

⁶Department for Thoracic-Surgery and Institute of Pathology and Molecular Pa, Bundeswehrkrankenhaus Ulm, Ulm, Germany

Find articles by Wenzel, W. in: PubMed | Google Scholar

¹Department of Infectious Diseases, Respiratory Medicine and Critical Care, Charité - Universitätsmedizin Berlin, Berlin, Germany

²Institute for Medical Informatics, Statistics, and Epidemiology, Universität Leipzig, ScaDS.AI, Leipzig, Germany

³Department of Internal Medicine and Department of Microbiology and Hospital, Bundeswehrkrankenhaus Berlin, Berlin, Germany

⁴Institute of Functional Anatomy, Charité - Universitätsmedizin Berlin, Berlin, Germany

⁵Respiratory Diseases Clinic Heckeshorn, Helios Klinikum Emil von Behring GmbH, Berlin, Germany

⁶Department for Thoracic-Surgery and Institute of Pathology and Molecular Pa, Bundeswehrkrankenhaus Ulm, Ulm, Germany

Find articles by Zobel, C. in: PubMed | Google Scholar

¹Department of Infectious Diseases, Respiratory Medicine and Critical Care, Charité - Universitätsmedizin Berlin, Berlin, Germany

²Institute for Medical Informatics, Statistics, and Epidemiology, Universität Leipzig, ScaDS.AI, Leipzig, Germany

³Department of Internal Medicine and Department of Microbiology and Hospital, Bundeswehrkrankenhaus Berlin, Berlin, Germany

⁴Institute of Functional Anatomy, Charité - Universitätsmedizin Berlin, Berlin, Germany

⁵Respiratory Diseases Clinic Heckeshorn, Helios Klinikum Emil von Behring GmbH, Berlin, Germany

⁶Department for Thoracic-Surgery and Institute of Pathology and Molecular Pa, Bundeswehrkrankenhaus Ulm, Ulm, Germany

Find articles by Nouailles, G. in: PubMed | Google Scholar

J Clin Invest. https://doi.org/10.1172/JCI192454.
Copyright © 2025, Hoffmann et al. This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Published November 18, 2025 - Version history

Mechanical strain exacerbates Pseudomonas infection in an organoid-based pneumonia-on-a-chip model

Karen Hoffmann,¹ Ulrike Behrendt,¹ Peter Pennitz,¹ Holger Kirsten,² Jessica Pohl,³ Elena Lopez-Rodriguez,⁴ Chantal Weissfuss,¹ Jens Kollmeier,⁵ Mario Tönnies,⁵ Sebastian Brill,⁶ Konrad Steinestel,⁶ Martin Witzenrath,¹ Werner Wenzel,³ Christian Zobel,³ and Geraldine Nouailles¹

Article tools

Metrics

Go to

Mechanical strain exacerbates Pseudomonas infection in an organoid-based pneumonia-on-a-chip model

Karen Hoffmann,1 Ulrike Behrendt,1 Peter Pennitz,1 Holger Kirsten,2 Jessica Pohl,3 Elena Lopez-Rodriguez,4 Chantal Weissfuss,1 Jens Kollmeier,5 Mario Tönnies,5 Sebastian Brill,6 Konrad Steinestel,6 Martin Witzenrath,1 Werner Wenzel,3 Christian Zobel,3 and Geraldine Nouailles1

Article tools

Metrics

Go to

Sign up for email alerts

Karen Hoffmann,¹ Ulrike Behrendt,¹ Peter Pennitz,¹ Holger Kirsten,² Jessica Pohl,³ Elena Lopez-Rodriguez,⁴ Chantal Weissfuss,¹ Jens Kollmeier,⁵ Mario Tönnies,⁵ Sebastian Brill,⁶ Konrad Steinestel,⁶ Martin Witzenrath,¹ Werner Wenzel,³ Christian Zobel,³ and Geraldine Nouailles¹