Prevention of leukocyte migration to inflamed skin with a novel fluorosugar modifier of cutaneous lymphocyte-associated antigen
J. Clin. Invest. Charles J. Dimitroff, et al. 112:1008 doi:10.1172/JCI19220 [Go to this article.]

Figure 1
Parallel-plate flow chamber analysis of E- and P-selectin binding of murine Th cells following 4-F-GlcNAc treatment. Murine Th1 cells were generated from spleens of C57BL/6 wild-type and FucTVII-deficient mice. To assess the effects of 4-F-GlcNAc on the de novo synthesis of selectin ligands, Th1 cell cultures were first treated with neuraminidase (0.1 U/ml for 1 hour at 37°C) and then regrown for 30 hours in fresh medium containing PBS (diluent), 0.05 mM 4-F-GlcNAc, 0.23 mM swainsonine, or 1 mM GlcNAc (negative control). Cells were harvested, suspended in HBSS with 2 mM CaCl₂ and 10 mM HEPES, and perfused over human recombinant E-selectin–Ig chimera (a) or P-selectin–Ig chimera (b) in the parallel-plate flow chamber. Assessments of cell rolling were made at 1.5 dynes/cm² from the midpoint of the chamber viewing field (mean ± SEM from four fields per selectin spot and three different experiments). Rolling Th1 cells on E- or P-selectin–Ig were inhibitable with 0.5 mM EDTA, and rolling adhesions on human IgG–coated plastic were absent. *P < 0.001, Student’s paired t test. Neur, neuraminidase.