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A traditional herbal medicine enhances bilirubin clearance by activating the nuclear receptor CAR
Wendong Huang, Jun Zhang, David D. Moore
Published in Volume 113, Issue 1
J Clin Invest. 2004; 113(1):137–143 doi:10.1172/JCI18385
This article Copyright © 2004, The American Society for Clinical Investigation
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Figure 2

Yin Zhi Huang treatment stimulates bilirubin clearance in WT, but not CAR, knockout mice. (a) Groups of four WT or CAR–/– mice were gavaged with either saline (PBS), control (CO), or Yin Zhi Huang (YZH) decoction (10 ml/kg/day) for 3 days. On the fourth day, mice were subjected to an acute bilirubin clearance assay, and total serum bilirubin was determined as described. *P < 0.05 relative to the control mice. (b) Total liver RNA was prepared from the animals as in (a). Equivalent amounts of RNA were pooled from four individuals, and 15 μg of each RNA sample was used for Northern hybridization with different probes as indicated. (c) Four groups of humanized CAR mice were treated with either PBS or increasing concentrations of YZH (2.5, 5 or 10 ml/kg/day) for 3 days. An acute bilirubin clearance assay, as well as Northern hybridization with CYP2B10 and human CAR probes, was carried out.

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