Regulatory functions of CD8⁺CD28^– T cells in an autoimmune disease model
J. Clin. Invest. Nader Najafian, et al. 112:1037 doi:10.1172/JCI17935 [Go to this article.]

Figure 1
Screening of CD28^–/–CD8^–/– mice by flow cytometry and PCR. A representative example of screening of CD28^–/–CD8^–/– mice and WT mice is shown. Cells were stained and analyzed by FACS for expression of CD8 and CD28. For the PCR screening, genomic DNA was extracted from tails of animals. In the CD8 PCR sample, homozygous samples produced a single 343-bp band, and WT mice produced a single 265-bp band. Heterozygotes produced both bands. In the CD28 PCR sample, homozygous samples produced a single 740-bp band, and WT mice produced a single 600-bp band. Heterozygous mice produced both bands. All PCR experiments included a no-template control and control reactions using DNA from known heterozygous and WT samples. Neg, negative; FL1-H, fluorescence channel 1.