Prolactin modulates the naive B cell repertoire
J. Clin. Invest. Elena Peeva, et al. 111:275 doi:10.1172/JCI16530 [Go to this article.]

Figure 1
(a) Serum titers of anti-dsDNA antibodies. Fourteen R4A-γ2b BALB/c mice were injected subcutaneously with 100 μg of murine prolactin daily and 12 were injected with placebo daily for 4 weeks. Sera collected before initiation of treatment and weekly thereafter showed an increase in anti-DNA titer in placebo-treated mice. Anti-DNA reactivity assayed from serum obtained at the end of the treatment showed that prolactin-treated mice had significantly higher titers of anti-DNA antibody than placebo-treated mice (P = 0.023). OD 405, optical density at 405 nanometers. (b) Immunohistochemistry of kidney sections. Fourteen prolactin-treated and 12 placebo-treated R4A-γ2b BALB/c mice were sacrificed after 4 weeks of treatment. The kidneys were stained for IgG deposition. Anti-DNA deposits were found in prolactin-treated mice but not in placebo-treated mice. Representative sections from mice treated with prolactin or placebo are shown. Magnification, ×40.