In vivo derivation of glucose-competent pancreatic endocrine cells from bone marrow without evidence of cell fusion
J. Clin. Invest. Andreea Ianus, et al. 111:843 doi:10.1172/JCI16502 [Go to this article.]

Figure 5
RT-PCR of isolated and FACS-sorted cells from experiment 1. (a) Peripheral blood nucleated cells (PBNCs) of recipient mice express cyclophilin (lane 1) as well as CD45 (lane 2), and EGFP-expressing cells derived from pancreatic islets express cyclophilin (lane 3), lack CD45 (lane 4), and express insulin (lane 5). (b) EGFP-expressing cells isolated from pancreatic islets do not express the stem cell marker oct-3/4. Lane 1: Positive control oct-3/4 plasmid. Bone marrow cells express cyclophilin (lane 2) and oct-3/4 (lane 3), and EGFP-expressing cells from pancreatic islets express cyclophilin (lane 4) but not oct-3/4 (lane 5). (c) EGFP-expressing cells isolated from pancreatic islets express cyclophilin (lane 1), insulin I (lane 2), IPF-1 (lane 4), and HNF3β (lane 5). Lane 3 is empty. (d) EGFP-expressing cells derived from pancreatic islets express cyclophilin (lane 1), insulin II (lane 2), insulin I (lane 3), GLUT2 (lane 4), HNF1α (lane 5), HNF1β (lane 6), and PAX6 (lane 7).