A novel dispersin protein in enteroaggregative Escherichia coli
J. Clin. Invest. Jalaluddin Sheikh, et al. 110:1329 doi:10.1172/JCI16172 [
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Figure 7Role of
aap in epithelial cell adherence. Assays were performed for 3 hours in DMEM–0.5% glucose with 0.2 mM IPTG at 37°C in atmosphere. (
a) 042; (
b) 042
aap; (
c) 042
aap(pAap). (
d and
e) The standard HEp-2 cell adherence assay was performed using strain 042
aap, but with pure Aap (
d) or pure BSA (
e) added concurrently with the bacterial inoculum. Each protein was added to a final concentration of 0.1 mg/ml. (
f) Quantitation of HEp-2 cell adherence on confluent monolayers growing in DMEM without IPTG (042 and 042
aap) or with indicated IPTG concentration [042
aap(pAap)]. Bacteria were removed from glass using 1% Triton X-100 and quantitated by viable count. Assays were performed in triplicate, and means are illustrated in histograms with error bars indicating SE. *
P < 0.05 vs. 042 by one-tailed
t test of log-transformed data; **
P < 0.05 vs. 042
aap by one-tailed
t test of log-transformed data.
