Histone deacetylase inhibitors modulate renal disease in the MRL-lpr/lpr mouse
J. Clin. Invest. Nilamadhab Mishra, et al. 111:539 doi:10.1172/JCI16153 [Go to this article.]

Figure 5
Western immunoblot analysis of acetylated histones H3 and H4 in 24-week-old MRL-lpr/lpr splenocytes. Histones were isolated by acid extraction from cells cultured with different doses of TSA or SAHA for 18 hours. Acetylation was detected using anti–acetylated H3 and H4 Ab’s. A parallel gel stained with Coomassie blue is shown as a control for protein loading in each lane (66–68).