James O. Park, Carlos A. Lopez, Vinay K. Gupta, Charles K. Brown, Helena J. Mauceri, Thomas E. Darga, Abdullah Manan, Samuel Hellman, Mitchell C. Posner, Donald W. Kufe, Ralph R. Weichselbaum
In vitro measurement of TNF-α protein. TNF-α production by Ad.Egr.TNF.11D-infected cells exposed to IR (5 Gy) or cisplatin (5 μM) was measured using ELISA. Significant increases in levels of TNF-α protein were detected at 24, 48, and 72 hours following exposure to Ad.Egr.TNF.11D plus IR (P < 0.001) and Ad.Egr.TNF.11D plus cisplatin (P < 0.001) compared with vector alone in Seg-1 cultures (a) and PROb cultures (b). Data are reported as mean ± SEM of three independent experiments.