Effects of reduced mucus oxygen concentration in airway Pseudomonas infections of cystic fibrosis patients
J. Clin. Invest. Dieter Worlitzsch, et al. 109:317 doi:10.1172/JCI13870 [
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Figure 4Growth and alginate production of
P. aeruginosa under aerobic versus anaerobic conditions. (
a) Growth of
P. aeruginosa in NL or CF ASL under aerobic or anaerobic conditions. Two strains, PAO1 (black bars) and ATCC 700829 (gray bars), were inoculated (∼100–200 bacteria, dashed line) in 30 μl NL or CF ASL and number of bacteria quantitated 72 hours later. The results presented are from a single representative experiment of three performed. The differences in the CFU/ml in the three experiments were less than 0.3 log 10. (
b) Immunofluorescence detection of alginate production by PAO1 after aerobic (8 hours; left) or anaerobic (12 hours; right) conditions (magnification, ×1,000; bars, 10 μm). (
c) Alginate production of PAO1 by the carbazole assay after growth under anaerobic (black bar) or aerobic (white bar) conditions for 4 days without added nitrate. *
P < 0.05. (
d) Alginate production per microgram bacterial protein mass of PAO1 as a function of the added NO
3– to PIA under aerobic (white bars) and anaerobic (black bars) conditions. (
e) pO
2 (filled squares) in an aerobically growing suspension of
P. aeruginosa (open triangles) as a function of time (hours). (
f) Mathematical analysis of depths from air-mucus interface at which pO
2 becomes zero for simulated mucus masses/plaques containing different concentrations of
P. aeruginosa bacteria (colony-forming units per milliliter).
