Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
Top
  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal
  • Top
  • Abstract
  • Version history
  • Article usage
  • Citations to this article

Advertisement

Research Article Free access | 10.1172/JCI117118

Bronchial biopsy evidence for leukocyte infiltration and upregulation of leukocyte-endothelial cell adhesion molecules 6 hours after local allergen challenge of sensitized asthmatic airways.

S Montefort, C Gratziou, D Goulding, R Polosa, D O Haskard, P H Howarth, S T Holgate, and M P Carroll

Department of Immunopharmacology, University Medicine, Southampton General Hospital, United Kingdom.

Find articles by Montefort, S. in: PubMed | Google Scholar

Department of Immunopharmacology, University Medicine, Southampton General Hospital, United Kingdom.

Find articles by Gratziou, C. in: PubMed | Google Scholar

Department of Immunopharmacology, University Medicine, Southampton General Hospital, United Kingdom.

Find articles by Goulding, D. in: PubMed | Google Scholar

Department of Immunopharmacology, University Medicine, Southampton General Hospital, United Kingdom.

Find articles by Polosa, R. in: PubMed | Google Scholar

Department of Immunopharmacology, University Medicine, Southampton General Hospital, United Kingdom.

Find articles by Haskard, D. in: PubMed | Google Scholar

Department of Immunopharmacology, University Medicine, Southampton General Hospital, United Kingdom.

Find articles by Howarth, P. in: PubMed | Google Scholar

Department of Immunopharmacology, University Medicine, Southampton General Hospital, United Kingdom.

Find articles by Holgate, S. in: PubMed | Google Scholar

Department of Immunopharmacology, University Medicine, Southampton General Hospital, United Kingdom.

Find articles by Carroll, M. in: PubMed | Google Scholar

Published April 1, 1994 - More info

Published in Volume 93, Issue 4 on April 1, 1994
J Clin Invest. 1994;93(4):1411–1421. https://doi.org/10.1172/JCI117118.
© 1994 The American Society for Clinical Investigation
Published April 1, 1994 - Version history
View PDF
Abstract

We have examined the mucosal changes occurring in bronchial biopsies from six atopic asthmatics 5-6 h after local endobronchial allergen challenge and compared them with biopsies from saline-challenged segments from the same subjects at the same time point. All the subjects developed localized bronchoconstriction in the allergen-challenged segment and had a decrease in forced expiratory volume in 1 s (FEV1) (P < 0.01) and a decrease in their methacholine provocative concentration of agonist required to reduce FEV1 from baseline by 20% (P < 0.05) 24 h postchallenge. At 6 h we observed an increase in neutrophils (P = 0.03), eosinophils (P = 0.025), mast cells (P = 0.03), and CD3+ lymphocytes (P = 0.025), but not in CD4+ or CD8+ lymphocyte counts. We also detected an increase in endothelial intercellular adhesion molecule type 1 (P < 0.05) and E-selectin (P < 0.005), but not vascular cell adhesion molecule type 1 expression with a correlative increase in submucosal and epithelial LFA+ leucocytes (P < 0.01). Thus, in sensitized asthmatics, local endobronchial allergen instillation leads to an increased inflammatory cell infiltrate of the airway mucosa that involves upregulation of specific adhesion molecules expressed on the microvasculature.

Images.

Browse pages

Click on an image below to see the page. View PDF of the complete article

icon of scanned page 1411
page 1411
icon of scanned page 1412
page 1412
icon of scanned page 1413
page 1413
icon of scanned page 1414
page 1414
icon of scanned page 1415
page 1415
icon of scanned page 1416
page 1416
icon of scanned page 1417
page 1417
icon of scanned page 1418
page 1418
icon of scanned page 1419
page 1419
icon of scanned page 1420
page 1420
icon of scanned page 1421
page 1421
Version history
  • Version 1 (April 1, 1994): No description

Article tools

  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal

Metrics

  • Article usage
  • Citations to this article

Go to

  • Top
  • Abstract
  • Version history
Advertisement
Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts