Advertisement

Amendment history:

Research Article Free access | 10.1172/JCI117079

Cloning, characterization, and chromosomal mapping of human aquaporin of collecting duct.

S Sasaki, K Fushimi, H Saito, F Saito, S Uchida, K Ishibashi, M Kuwahara, T Ikeuchi, K Inui, and K Nakajima

Second Department of Internal Medicine, School of Medicine, Tokyo Medical and Dental University, Japan.

Find articles by Sasaki, S. in: PubMed | Google Scholar

Second Department of Internal Medicine, School of Medicine, Tokyo Medical and Dental University, Japan.

Find articles by Fushimi, K. in: PubMed | Google Scholar

Second Department of Internal Medicine, School of Medicine, Tokyo Medical and Dental University, Japan.

Find articles by Saito, H. in: PubMed | Google Scholar

Second Department of Internal Medicine, School of Medicine, Tokyo Medical and Dental University, Japan.

Find articles by Saito, F. in: PubMed | Google Scholar

Second Department of Internal Medicine, School of Medicine, Tokyo Medical and Dental University, Japan.

Find articles by Uchida, S. in: PubMed | Google Scholar

Second Department of Internal Medicine, School of Medicine, Tokyo Medical and Dental University, Japan.

Find articles by Ishibashi, K. in: PubMed | Google Scholar

Second Department of Internal Medicine, School of Medicine, Tokyo Medical and Dental University, Japan.

Find articles by Kuwahara, M. in: PubMed | Google Scholar

Second Department of Internal Medicine, School of Medicine, Tokyo Medical and Dental University, Japan.

Find articles by Ikeuchi, T. in: PubMed | Google Scholar

Second Department of Internal Medicine, School of Medicine, Tokyo Medical and Dental University, Japan.

Find articles by Inui, K. in: PubMed | Google Scholar

Second Department of Internal Medicine, School of Medicine, Tokyo Medical and Dental University, Japan.

Find articles by Nakajima, K. in: PubMed | Google Scholar

Published March 1, 1994 - More info

Published in Volume 93, Issue 3 on March 1, 1994
J Clin Invest. 1994;93(3):1250–1256. https://doi.org/10.1172/JCI117079.
© 1994 The American Society for Clinical Investigation
Published March 1, 1994 - Version history
View PDF
Abstract

We recently cloned a cDNA of the collecting duct apical membrane water channel of rat kidney, which is important for the formation of concentrated urine (Fushima, K., S. Uchida, Y. Hara, Y. Hirata, F. Marumo, and S. Sasaki. 1993. Nature [Lond.]. 361:549-552). Since urine concentrating ability varies among mammalian species, we examined whether an homologous protein is present in human kidney. By screening a human kidney cDNA library, we isolated a cDNA clone, designated human aquaporin of collecting duct (hAQP-CD), that encodes a 271-amino acid protein with 91% identity to rat AQP-CD. mRNA expression of hAQP-CD was predominant in the kidney medulla compared with the cortex, immunohistochemical staining of hAQP-CD was observed only in the collecting duct cells, and the staining was dominant in the apical domain. Functional expression study in Xenopus oocytes confirmed that hAQP-CD worked as a water channel. Western blot analysis of human kidney medulla indicated that the molecular mass of hAQP-CD is 29 kD, which is the same mass expected from the amino acid sequence. Chromosomal mapping of the hAQP-CD gene assigned its location to chromosome 12q13. These results could be important for future studies of the pathophysiology of human urinary concentration mechanisms in normal and abnormal states.

Images.

Browse pages

Click on an image below to see the page. View PDF of the complete article

Version history
  • Version 1 (March 1, 1994): No description
Advertisement
Advertisement