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Effect of denervation on the expression of two glucose transporter isoforms in rat hindlimb muscle.

N E Block, D R Menick, K A Robinson, and M G Buse

Division of Endocrinology, Metabolism and Nutrition, Gazes Cardiac Research Institute, Medical University of South Carolina, Charleston 29425.

Find articles by Block, N. in: JCI | PubMed | Google Scholar

Division of Endocrinology, Metabolism and Nutrition, Gazes Cardiac Research Institute, Medical University of South Carolina, Charleston 29425.

Find articles by Menick, D. in: JCI | PubMed | Google Scholar

Division of Endocrinology, Metabolism and Nutrition, Gazes Cardiac Research Institute, Medical University of South Carolina, Charleston 29425.

Find articles by Robinson, K. in: JCI | PubMed | Google Scholar

Division of Endocrinology, Metabolism and Nutrition, Gazes Cardiac Research Institute, Medical University of South Carolina, Charleston 29425.

Find articles by Buse, M. in: JCI | PubMed | Google Scholar

First published November 1, 1991 - More info

Published in Volume 88, Issue 5 (November 1, 1991)
J Clin Invest. 1991;88(5):1546–1552. doi:10.1172/JCI115465.
Copyright © 1991, The American Society for Clinical Investigation.
Published November 1, 1991

Research Article

Abstract

Denervation rapidly (within 24 h) induces insulin resistance of several insulin-responsive pathways in skeletal muscle, including glucose transport; resistance is usually maximal by 3 d. We examined the effect of denervation on the expression of two glucose transporter isoforms (GLUT-1 and GLUT-4) in rat hindlimb muscle; GLUT-4 is the predominant species in muscle. 1 d postdenervation, GLUT-1 and GLUT-4 mRNA and protein concentrations were unchanged. 3 and 7 d postdenervation, GLUT-4 mRNA and protein (per microgram DNA) were decreased by 50%. The minor isoform, GLUT-1 mRNA increased by approximately 500 and approximately 100%, respectively, on days 3 and 7 while GLUT-1 protein increased by approximately 60 and approximately 100%. The data suggest that the insulin resistance of glucose transport early after denervation does not reflect a decrease in total glucose transporter number; however, decreased GLUT-4 expression may contribute to its increased severity after 3 d. Parallel decreases in GLUT-4 mRNA and GLUT-4 protein postdenervation are consistent with pretranslational regulation; GLUT-1 expression may be regulated pre- and posttranslationally. The cell type(s) which overexpress GLUT-1 postdenervation need to be identified. Nervous stimuli and/or contractile activity may modulate the expression of GLUT-1 and GLUT-4 in skeletal muscle tissue.

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