Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
Top
  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal
  • Top
  • Abstract
  • Version history
  • Article usage
  • Citations to this article

Advertisement

Research Article Free access | 10.1172/JCI113622

An in vitro human muscle preparation suitable for metabolic studies. Decreased insulin stimulation of glucose transport in muscle from morbidly obese and diabetic subjects.

G L Dohm, E B Tapscott, W J Pories, D J Dabbs, E G Flickinger, D Meelheim, T Fushiki, S M Atkinson, C W Elton, and J F Caro

Department of Biochemistry, School of Medicine, East Carolina University, Greenville, North Carolina 27858.

Find articles by Dohm, G. in: PubMed | Google Scholar

Department of Biochemistry, School of Medicine, East Carolina University, Greenville, North Carolina 27858.

Find articles by Tapscott, E. in: PubMed | Google Scholar

Department of Biochemistry, School of Medicine, East Carolina University, Greenville, North Carolina 27858.

Find articles by Pories, W. in: PubMed | Google Scholar

Department of Biochemistry, School of Medicine, East Carolina University, Greenville, North Carolina 27858.

Find articles by Dabbs, D. in: PubMed | Google Scholar

Department of Biochemistry, School of Medicine, East Carolina University, Greenville, North Carolina 27858.

Find articles by Flickinger, E. in: PubMed | Google Scholar

Department of Biochemistry, School of Medicine, East Carolina University, Greenville, North Carolina 27858.

Find articles by Meelheim, D. in: PubMed | Google Scholar

Department of Biochemistry, School of Medicine, East Carolina University, Greenville, North Carolina 27858.

Find articles by Fushiki, T. in: PubMed | Google Scholar

Department of Biochemistry, School of Medicine, East Carolina University, Greenville, North Carolina 27858.

Find articles by Atkinson, S. in: PubMed | Google Scholar

Department of Biochemistry, School of Medicine, East Carolina University, Greenville, North Carolina 27858.

Find articles by Elton, C. in: PubMed | Google Scholar

Department of Biochemistry, School of Medicine, East Carolina University, Greenville, North Carolina 27858.

Find articles by Caro, J. in: PubMed | Google Scholar

Published August 1, 1988 - More info

Published in Volume 82, Issue 2 on August 1, 1988
J Clin Invest. 1988;82(2):486–494. https://doi.org/10.1172/JCI113622.
© 1988 The American Society for Clinical Investigation
Published August 1, 1988 - Version history
View PDF
Abstract

We have developed an in vitro muscle preparation suitable for metabolic studies with human muscle tissue and have investigated the effects of obesity and non-insulin-dependent diabetes mellitus (NIDDM) on glucose transport. Transport of 3-O-methylglucose and 2-deoxyglucose was stimulated approximately twofold by insulin in muscle from normal nonobese subjects and stimulation occurred in the normal physiological range of insulin concentrations. In contrast to insulin stimulation of 3-O-methylglucose and 2-deoxyglucose transport in muscle from normal, nonobese subjects, tissue from morbidly obese subjects, with or without NIDDM, were not responsive to insulin. Maximal 3-O-methylglucose transport was lower in muscle of obese than nonobese subjects. Morbidly obese patients, with or without NIDDM, have a severe state of insulin resistance in glucose transport. The novel in vitro human skeletal muscle preparation herein described should be useful in investigating the mechanism of this insulin resistance.

Images.

Browse pages

Click on an image below to see the page. View PDF of the complete article

icon of scanned page 486
page 486
icon of scanned page 487
page 487
icon of scanned page 488
page 488
icon of scanned page 489
page 489
icon of scanned page 490
page 490
icon of scanned page 491
page 491
icon of scanned page 492
page 492
icon of scanned page 493
page 493
icon of scanned page 494
page 494
Version history
  • Version 1 (August 1, 1988): No description

Article tools

  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal

Metrics

  • Article usage
  • Citations to this article

Go to

  • Top
  • Abstract
  • Version history
Advertisement
Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts